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Team:Groningen/Labwork/9 July 2013
From 2013.igem.org
(Difference between revisions)
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<h2>Mirjam</h2> | <h2>Mirjam</h2> | ||
| - | <br/>PCR made for silk without strep-tags. | + | <br/><a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> made for silk without strep-tags. |
| - | <br/> | + | <br/><a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> run for 22 min 90V. |
<br/>Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V. | <br/>Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V. | ||
<br/>Cut wrong bands from gel. So a new PCR is made. | <br/>Cut wrong bands from gel. So a new PCR is made. | ||
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Cells need to grow over night. | Cells need to grow over night. | ||
| - | 15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low) | + | 15:00 Did a <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> in order to get more silk gene to cut out of gel(last one was cut to low) |
Latest revision as of 10:02, 30 July 2013
Mirjam
PCR made for silk without strep-tags.
gel run for 22 min 90V.
Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
Cut wrong bands from gel. So a new PCR is made.
