Team:Groningen/Modelling/3 September 2013
From 2013.igem.org
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PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively. | PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively. | ||
<br>The presence of the expected products was checked using gel electrophoresis (agarose 0.8%). | <br>The presence of the expected products was checked using gel electrophoresis (agarose 0.8%). | ||
+ | <br> | ||
+ | <br>pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI. | ||
+ | <br>The samples were checked using gel electrophoresis (agarose 0.8%). | ||
</div> | </div> |
Revision as of 10:30, 3 September 2013
Claudio
PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively.The presence of the expected products was checked using gel electrophoresis (agarose 0.8%).
pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI.
The samples were checked using gel electrophoresis (agarose 0.8%).