Team:Groningen/Modelling/3 September 2013

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PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively.
PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively.
<br>The presence of the expected products was checked using gel electrophoresis (agarose 0.8%).
<br>The presence of the expected products was checked using gel electrophoresis (agarose 0.8%).
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<br>pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI.
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<br>The samples were checked using gel electrophoresis (agarose 0.8%).
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Revision as of 10:30, 3 September 2013

Claudio

PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively.
The presence of the expected products was checked using gel electrophoresis (agarose 0.8%).

pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI.
The samples were checked using gel electrophoresis (agarose 0.8%).