Team:Groningen/SchematicOverview

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Revision as of 02:33, 5 October 2013
The spider silk gene and the Pdes (heat sensing) promoter with CheY (movement) gene downstream of it are put in our backbone, and inserted into the Bacillus subtilis genome.
The transformed B. subtilis is then put in a bath with a heated implant
The secreted spider silk with strep-tag attaches to the implant its streptavidin coating. The coating is processed with evaporation, treated with kosmotropic ions and heated thoroughly
The implant is now coated by the Coating GEMs and ready for use!