Team:Groningen/protocols/Digestion
From 2013.igem.org
(Difference between revisions)
Line 59: | Line 59: | ||
<br>All the reagents are added following the order listed in the table above. | <br>All the reagents are added following the order listed in the table above. | ||
<br>After the reaction is ready mix the content of the tube and spin it down. | <br>After the reaction is ready mix the content of the tube and spin it down. | ||
- | <br>The tubes are incubated for 1h at 37& | + | <br>The tubes are incubated for 1h at 37°g. |
<br> | <br> | ||
<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf | <br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf |
Revision as of 09:52, 26 July 2013
Digestion
Materials:
- H2O
- 1.5ml tubes
- Ligation buffer
- Restriction enzymes
- DNA
Reaction:
Component | 30μl reaction | Final concentration |
H2O | up to 30μl | |
Buffer 10x | 3μl | 1x |
DNA | 4μl | up to 1μg |
Enzyme/s | 0.5μl |
All the reagents are added following the order listed in the table above.
After the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated for 1h at 37°g.
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf