Team:Groningen/protocols/Digestion

From 2013.igem.org

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<br>All the reagents are added following the order listed in the table above.
<br>All the reagents are added following the order listed in the table above.
-
<br>After the reaction is ready mix the content of the tube and spin it down.
+
<br>When the reaction mix is reaAfter the reaction is ready mix the content of the tube and spin it down.
-
<br>The tubes are incubated for 1h at 37&deg;C.
+
<br>The tubes are incubated at 37&deg;C for 1h.
<br>
<br>
<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf
<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf

Revision as of 14:03, 27 July 2013

Digestion

Fast digest

Materials:

  • MQ water
  • 1.5ml tubes
  • Fast digestion buffer
  • Restriction enzymes
  • DNA

Reaction:

Compound 20µl Final concentration
MQ water up to 20µl
10x buffer 2µl 1x
restriction enzyme 1µl 1U
DNA 4µl up to 1µl

All the reagents are added following the order listed in the table above.
When the reaction mix is reaAfter the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated at 37°C for 1h.

Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf