Team:Groningen/protocols/Digestion
From 2013.igem.org
(Difference between revisions)
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<br>All the reagents are added following the order listed in the table above. | <br>All the reagents are added following the order listed in the table above. | ||
- | <br> | + | <br>When the reaction mix is reaAfter the reaction is ready mix the content of the tube and spin it down. |
- | <br>The tubes are incubated | + | <br>The tubes are incubated at 37°C for 1h. |
<br> | <br> | ||
<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf | <br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf |
Revision as of 14:03, 27 July 2013
Digestion
Fast digest
Materials:
- MQ water
- 1.5ml tubes
- Fast digestion buffer
- Restriction enzymes
- DNA
Reaction:
Compound | 20µl | Final concentration |
---|---|---|
MQ water | up to 20µl | |
10x buffer | 2µl | 1x |
restriction enzyme | 1µl | 1U |
DNA | 4µl | up to 1µl |
All the reagents are added following the order listed in the table above.
When the reaction mix is reaAfter the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated at 37°C for 1h.
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf