Team:Groningen/protocols/Digestion

From 2013.igem.org

(Difference between revisions)
Line 50: Line 50:
   <tr>
   <tr>
-
     <td>10x buffer</td>
+
     <td>10x fast digestion buffer</td>
     <td>2&micro;l</td>
     <td>2&micro;l</td>
     <td>1x</td>
     <td>1x</td>
Line 70: Line 70:
-
<br>When the reaction mix is ready, the concent is mixed an incubated at 37&deg;C for 1h.
+
<br>When the reaction mix is ready, the content is mixed an incubated at 37&deg;C for 1h.
<br>
<br>
<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf
<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf

Revision as of 14:11, 27 July 2013

Digestion

Fast digest

Materials:

  • MQ water
  • 1.5ml tubes
  • Fast digestion buffer
  • Restriction enzymes
  • DNA

Reaction:

Compound 20µl Final concentration
MQ water up to 20µl
10x fast digestion buffer 2µl 1x
restriction enzyme(s) 1µl 1U
DNA 4µl up to 1µl

When the reaction mix is ready, the content is mixed an incubated at 37°C for 1h.

Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf