Team:Groningen/protocols/Digestion
From 2013.igem.org
(Difference between revisions)
Line 44: | Line 44: | ||
</tr> | </tr> | ||
<tr><td>Buffer 10x</td> | <tr><td>Buffer 10x</td> | ||
- | <td>3</td> | + | <td>3μl</td> |
<td>1x</td> | <td>1x</td> | ||
</tr> | </tr> | ||
Line 52: | Line 52: | ||
</tr> | </tr> | ||
<tr><td>Enzyme/s</td> | <tr><td>Enzyme/s</td> | ||
- | <td>0.5</td> | + | <td>0.5μl</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | <br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf | ||
</div> | </div> |
Revision as of 09:48, 26 July 2013
Digestion
Materials:
- H2O
- 1.5ml tubes
- Ligation buffer
- Restriction enzymes
- DNA
Reaction:
Component | 30μl reaction | Final concentration |
H2O | up to 30μl | |
Buffer 10x | 3μl | 1x |
DNA | 4μl | up to 1μg |
Enzyme/s | 0.5μl |
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf