Team:Groningen/protocols/Digestion

From 2013.igem.org

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<tr><td>Buffer 10x</td>
<tr><td>Buffer 10x</td>
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<td>1x</td>
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<tr><td>Enzyme/s</td>
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<td>0.5</td>
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<td>0.5&#956;l</td>
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<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf
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Revision as of 09:48, 26 July 2013

Digestion

Materials:

  • H2O
  • 1.5ml tubes
  • Ligation buffer
  • Restriction enzymes
  • DNA

Reaction:

Component 30μl reaction Final concentration
H2O up to 30μl
Buffer 10x 3μl 1x
DNA 4μl up to 1μg
Enzyme/s 0.5μl

Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf