Team:Groningen/protocols/Digestion

From 2013.igem.org

(Difference between revisions)
Line 28: Line 28:
<li>H<sub>2</sub>O</li>
<li>H<sub>2</sub>O</li>
<li>1.5ml tubes</li>
<li>1.5ml tubes</li>
-
<li>Ligation buffer</li>
+
<li>Digestion buffer</li>
<li>Restriction enzymes</li>
<li>Restriction enzymes</li>
<li>DNA</li>
<li>DNA</li>

Revision as of 09:57, 26 July 2013

Digestion

Materials:

  • H2O
  • 1.5ml tubes
  • Digestion buffer
  • Restriction enzymes
  • DNA

Reaction:

Component 30μl reaction Final concentration
H2O up to 30μl
Buffer 10x 3μl 1x
DNA 4μl up to 1μg
Enzyme/s 0.5μl

All the reagents are added following the order listed in the table above.
After the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated for 1h at 37°C.

Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf