Team:Groningen/protocols/Digestion
From 2013.igem.org
(Difference between revisions)
Line 28: | Line 28: | ||
<li>H<sub>2</sub>O</li> | <li>H<sub>2</sub>O</li> | ||
<li>1.5ml tubes</li> | <li>1.5ml tubes</li> | ||
- | <li> | + | <li>Digestion buffer</li> |
<li>Restriction enzymes</li> | <li>Restriction enzymes</li> | ||
<li>DNA</li> | <li>DNA</li> |
Revision as of 09:57, 26 July 2013
Digestion
Materials:
- H2O
- 1.5ml tubes
- Digestion buffer
- Restriction enzymes
- DNA
Reaction:
Component | 30μl reaction | Final concentration |
H2O | up to 30μl | |
Buffer 10x | 3μl | 1x |
DNA | 4μl | up to 1μg |
Enzyme/s | 0.5μl |
All the reagents are added following the order listed in the table above.
After the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated for 1h at 37°C.
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf