Team:Groningen/protocols/Digestion
From 2013.igem.org
(Difference between revisions)
Line 23: | Line 23: | ||
<h1>Digestion</h1> | <h1>Digestion</h1> | ||
+ | |||
+ | <h2>Fast digest</h2> | ||
<h3>Materials:</h3> | <h3>Materials:</h3> | ||
<ul type="square"> | <ul type="square"> | ||
- | <li> | + | <li>MQ water</li> |
<li>1.5ml tubes</li> | <li>1.5ml tubes</li> | ||
- | <li> | + | <li>Fast digestion buffer</li> |
<li>Restriction enzymes</li> | <li>Restriction enzymes</li> | ||
<li>DNA</li> | <li>DNA</li> | ||
</ul> | </ul> | ||
+ | |||
+ | {| class="wikitable" style="text-align: center; color: green;" | ||
+ | |Orange | ||
+ | |Apple | ||
+ | |12,333.00 | ||
+ | |- | ||
+ | |Bread | ||
+ | |Pie | ||
+ | |500.00 | ||
+ | |- | ||
+ | |Butter | ||
+ | |Ice cream | ||
+ | |1.00 | ||
+ | |} | ||
<h3>Reaction:</h3> | <h3>Reaction:</h3> | ||
+ | {| class="wikitable" style="text-align: center;" | ||
+ | |Component | ||
+ | |20µl | ||
+ | |Final concentration | ||
+ | |- | ||
+ | |Bread | ||
+ | |Pie | ||
+ | |500.00 | ||
+ | |- | ||
+ | |Butter | ||
+ | |Ice cream | ||
+ | |1.00 | ||
+ | |} | ||
+ | |||
<table border = "1"> | <table border = "1"> | ||
<tr><td>Component</td> | <tr><td>Component</td> |
Revision as of 13:22, 27 July 2013
Digestion
Fast digest
Materials:
- MQ water
- 1.5ml tubes
- Fast digestion buffer
- Restriction enzymes
- DNA
Reaction:
{| class="wikitable" style="text-align: center;" |Component |20µl |Final concentration |- |Bread |Pie |500.00 |- |Butter |Ice cream |1.00 |}Component | 30μl reaction | Final concentration |
H2O | up to 30μl | |
Buffer 10x | 3μl | 1x |
DNA | 4μl | up to 1μg |
Enzyme/s | 1μl | 1U |
All the reagents are added following the order listed in the table above.
After the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated for 1h at 37°C.
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf