Team:Groningen/protocols/Digestion

From 2013.igem.org

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<h1>Digestion</h1>
<h1>Digestion</h1>
 +
 +
<h2>Fast digest</h2>
<h3>Materials:</h3>
<h3>Materials:</h3>
<ul type="square">
<ul type="square">
-
<li>H<sub>2</sub>O</li>
+
<li>MQ water</li>
<li>1.5ml tubes</li>
<li>1.5ml tubes</li>
-
<li>Digestion buffer</li>
+
<li>Fast digestion buffer</li>
<li>Restriction enzymes</li>
<li>Restriction enzymes</li>
<li>DNA</li>
<li>DNA</li>
</ul>
</ul>
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 +
{| class="wikitable" style="text-align: center; color: green;"
 +
|Orange
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|Apple
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|12,333.00
 +
|-
 +
|Bread
 +
|Pie
 +
|500.00
 +
|-
 +
|Butter
 +
|Ice cream
 +
|1.00
 +
|}
<h3>Reaction:</h3>
<h3>Reaction:</h3>
 +
{| class="wikitable" style="text-align: center;"
 +
|Component
 +
|20&microl
 +
|Final concentration
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|-
 +
|Bread
 +
|Pie
 +
|500.00
 +
|-
 +
|Butter
 +
|Ice cream
 +
|1.00
 +
|}
 +
<table border = "1">
<table border = "1">
<tr><td>Component</td>
<tr><td>Component</td>

Revision as of 13:22, 27 July 2013

Digestion

Fast digest

Materials:

  • MQ water
  • 1.5ml tubes
  • Fast digestion buffer
  • Restriction enzymes
  • DNA
{| class="wikitable" style="text-align: center; color: green;" |Orange |Apple |12,333.00 |- |Bread |Pie |500.00 |- |Butter |Ice cream |1.00 |}

Reaction:

{| class="wikitable" style="text-align: center;" |Component |20&microl |Final concentration |- |Bread |Pie |500.00 |- |Butter |Ice cream |1.00 |}
Component 30μl reaction Final concentration
H2O up to 30μl
Buffer 10x 3μl 1x
DNA 4μl up to 1μg
Enzyme/s 1μl 1U

All the reagents are added following the order listed in the table above.
After the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated for 1h at 37°C.

Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf