Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

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<h3>Procedure:</h3>
<h3>Procedure:</h3>
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All the DNA samples are mixed 1:1 ratio with Loading Dye 6x.
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All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
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<br>The samples are loaded on agarose gel 0.8% or 1.5% (diluted in TBE buffer 1x).
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<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb gene ruler of Fermentas.
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<br>Beside the samples the DNA ladder is loaded.
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<br>A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
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<br>90V electric potential is applied to the gel as long as the bands are apart from each other (30-45 minutes).
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<br>The gel is run in TBE buffer 1x.
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<br>The gel are run in TBE buffer 1x.
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</div>
</div>

Revision as of 17:26, 27 July 2013

Gel electrophoresis

Materials:

  • Power supply
  • Agarose gel
  • Tray
  • DNA samples
  • DNA Ladder

Procedure:

All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb gene ruler of Fermentas.
A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
The gel is run in TBE buffer 1x.