Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

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<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
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All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
+
<br>All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
<br>The gel is placed in TBE buffer 1x and a 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
<br>The gel is placed in TBE buffer 1x and a 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.

Revision as of 10:33, 28 July 2013

Gel electrophoresis

Materials:
  • Power supply
  • 0.8 or 1.5% agarose gel
  • Gel tray
  • 2x Loading Dye
  • DNA samples
  • DNA 1kb GeneRuler of Fermentas
Procedure:

- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.
All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
The gel is placed in TBE buffer 1x and a 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.