Gel electrophoresis

Materials:

• Power supply
• 0.8 or 1.5% agarose gel
• Gel tray
• 2x Loading Dye
• DNA samples
• DNA 1kb GeneRuler of Fermentas

Procedure:

- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands. All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
The gel is placed in TBE buffer 1x and a 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.