Team:Groningen/protocols/Motility assay

From 2013.igem.org

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     <th>compound</th>
     <th>compound</th>
     <th>amount</th>
     <th>amount</th>
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    <th>treatment</th>
 
   </tr>
   </tr>
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     <td>LB broth </td>
     <td>LB broth </td>
     <td>2 g</td>
     <td>2 g</td>
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    <td></td>
 
   </tr>
   </tr>
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     <td>agar</td>
     <td>agar</td>
     <td>0.4 g</td>
     <td>0.4 g</td>
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    <td></td>
 
   </tr>
   </tr>
</table>
</table>

Revision as of 00:20, 5 October 2013

Motility assay

Add 13 ml of the motility agar (0.4%) in every petri dish.
Inoculate the plates with the strain of interest, 10 µl with an OD600 of 0.4.
The plates are inoculated at different temperatures (25°C and 37°C) for 16 hours.
After 16 hours of growth, the diameter of the colony is measured.

Motility Agar 0.4% (100 ml)

compound amount
LB broth 2 g
agar 0.4 g