http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&feed=atom&action=historyTeam:HIT-Harbin/Experiments - Revision history2024-03-29T15:40:18ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=208227&oldid=prevSema1688 at 14:26, 27 September 20132013-09-27T14:26:13Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>4)We sent our whole device to companies for sequencing, but it failed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>4)We sent our whole device to companies for sequencing, but it failed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/71/HIT-Harbin_team.png"/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/71/HIT-Harbin_team.png"/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Fig: the happy moment when we saw the culture becoming red</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Fig: the happy moment when we saw the culture becoming red <ins class="diffchange diffchange-inline">for the first time</ins></p></div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=208129&oldid=prevSema1688 at 14:22, 27 September 20132013-09-27T14:22:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>4)We sent our whole device to companies for sequencing, but it failed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>4)We sent our whole device to companies for sequencing, but it failed.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2013/7/71/HIT-Harbin_team.png"/></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Fig: the happy moment when we saw the culture becoming red</p></ins></div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=207318&oldid=prevSema1688 at 13:49, 27 September 20132013-09-27T13:49:41Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/6/6b/HIT-Harbin_team_HIT.png"/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/6/6b/HIT-Harbin_team_HIT.png"/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Fig: Agarose electrophoresis for our parts and maker forming "HIT"</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Fig: Agarose electrophoresis for our parts and maker forming "HIT"</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Part A :Plasmid carrying Plac+RBS+hrpR+T</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Part A :Plasmid carrying Plac+RBS+hrpR+T</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Part B :Plasmid carrying Ptet+RBS+hrpS+T</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Part B :Plasmid carrying Ptet+RBS+hrpS+T</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Part C :Plasmid carrying PhrpL+RBS+RFP+T</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Part C :Plasmid carrying PhrpL+RBS+RFP+T</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"></div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=207290&oldid=prevSema1688 at 13:48, 27 September 20132013-09-27T13:48:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>Aug. 29~Sep. 15 Test of the device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>Aug. 29~Sep. 15 Test of the device</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>Sep. 16~25 Remaining experiments</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>Sep. 16~25 Remaining experiments</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div align="center"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2013/6/6b/HIT-Harbin_team_HIT.png"/></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Fig: Agarose electrophoresis for our parts and maker forming "HIT"</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Part A :Plasmid carrying Plac+RBS+hrpR+T</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Part B :Plasmid carrying Ptet+RBS+hrpS+T</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Part C :Plasmid carrying PhrpL+RBS+RFP+T</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=200827&oldid=prevSema1688 at 07:58, 27 September 20132013-09-27T07:58:14Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4 class="hashed"><span>2.Investigating the relationship between the concentration of RFP with that of IPTG</span></h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4 class="hashed"><span>2.Investigating the relationship between the concentration of RFP with that of IPTG</span></h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>1)Measuring absorbance of RFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>1)Measuring absorbance of RFP</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p></p>We grew bacteria without device and bacteria with our device in same volume until stationary phase. Taking bacteria without device as background, we measured the absorbance of bacteria with our device (the max absorption peak is 504nm).But absorbance in 504nm is higher than 1,which present a bad linear relation between absorbance and concentraton. RFP has absorption in 450nm,and absorbance is between 0.1 and 1(better linear relation)<del class="diffchange diffchange-inline">,occasionally</del>, we find a RFP standard curve under 450nm on the web. it was very lucky compared with our failure in testing our device. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p></p>We grew bacteria without device and bacteria with our device in same volume until stationary phase. Taking bacteria without device as background, we measured the absorbance of bacteria with our device (the max absorption peak is 504nm).But absorbance in 504nm is higher than 1,which present a bad linear relation between absorbance and concentraton. RFP has absorption in 450nm,and absorbance is between 0.1 and 1(better linear relation)<ins class="diffchange diffchange-inline">.Occasionally</ins>, we find a RFP standard curve under 450nm on the web. it was very lucky compared with our failure in testing our device. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Before the mensuration, we diluted the two groups according to table2. We took the mean of two measures as the useful data. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Before the mensuration, we diluted the two groups according to table2. We took the mean of two measures as the useful data. </div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=199415&oldid=prevSema1688 at 06:41, 27 September 20132013-09-27T06:41:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4 class="hashed"><span>2.Investigating the relationship between the concentration of RFP with that of IPTG</span></h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4 class="hashed"><span>2.Investigating the relationship between the concentration of RFP with that of IPTG</span></h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>1)Measuring absorbance of RFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>1)Measuring absorbance of RFP</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p></p>We grew bacteria without device and bacteria with our device in same volume until stationary phase. Taking bacteria without device as background, we measured the absorbance of bacteria with our device (the max absorption peak is 504nm).But absorbance in 504nm is higher than 1,which present a bad linear relation between absorbance and concentraton. RFP has absorption in 450nm,and absorbance is between 0.1 and 1(better linear relation),occasionally, we find a RFP standard curve under 450nm on the web. it was very lucky compared with our failure in <del class="diffchange diffchange-inline">test for </del>our device. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p></p>We grew bacteria without device and bacteria with our device in same volume until stationary phase. Taking bacteria without device as background, we measured the absorbance of bacteria with our device (the max absorption peak is 504nm).But absorbance in 504nm is higher than 1,which present a bad linear relation between absorbance and concentraton. RFP has absorption in 450nm,and absorbance is between 0.1 and 1(better linear relation),occasionally, we find a RFP standard curve under 450nm on the web. it was very lucky compared with our failure in <ins class="diffchange diffchange-inline">testing </ins>our device. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Before the mensuration, we diluted the two groups according to table2. We took the mean of two measures as the useful data. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Before the mensuration, we diluted the two groups according to table2. We took the mean of two measures as the useful data. </div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=199367&oldid=prevSema1688 at 06:39, 27 September 20132013-09-27T06:39:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4 class="hashed"><span>2.Investigating the relationship between the concentration of RFP with that of IPTG</span></h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4 class="hashed"><span>2.Investigating the relationship between the concentration of RFP with that of IPTG</span></h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>1)Measuring absorbance of RFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></p>1)Measuring absorbance of RFP</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p></p>We grew bacteria without device and bacteria with our device in same volume until stationary phase. Taking bacteria without device as background, we measured the absorbance of bacteria with our device (the max absorption peak is 504nm).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p></p>We grew bacteria without device and bacteria with our device in same volume until stationary phase. Taking bacteria without device as background, we measured the absorbance of bacteria with our device (the max absorption peak is 504nm)<ins class="diffchange diffchange-inline">.But absorbance in 504nm is higher than 1,which present a bad linear relation between absorbance and concentraton. RFP has absorption in 450nm,and absorbance is between 0.1 and 1(better linear relation),occasionally, we find a RFP standard curve under 450nm on the web. it was very lucky compared with our failure in test for our device</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Before the mensuration, we diluted the two groups according to table2. We took the mean of two measures as the useful data. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Before the mensuration, we diluted the two groups according to table2. We took the mean of two measures as the useful data. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Fig 6. The relationship between RFP concentration and absorbance</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Fig 6. The relationship between RFP concentration and absorbance<ins class="diffchange diffchange-inline">(OD450)</ins></p></div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=199087&oldid=prevSema1688 at 06:27, 27 September 20132013-09-27T06:27:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Fig 7. RFP standard curve obtain from the web,<a href="http://www.cellbiolabs.com/sites/default/files/AKR-122-rfp-elisa-kit.pdf">Click here</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Fig 7. RFP standard curve obtain from the web,<a href="http://www.cellbiolabs.com/sites/default/files/AKR-122-rfp-elisa-kit.pdf">Click here</a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p></p>Through the standard curve, we can convert the relative concentration to the absolute concentration, and finally get the relationship between IPTG concentration and RFP concentration.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p></p>Compared to crushing cells to separate RFP, our method is simpler and easy to practice. Moreover, our relative concentration curve is credible. If the standard curve is reliable, our calculated result of RFP will be precise.</ins></div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=199013&oldid=prevSema1688 at 06:23, 27 September 20132013-09-27T06:23:54Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Fig 7. RFP standard curve obtain from the web,<<del class="diffchange diffchange-inline">link </del>href="http://www.cellbiolabs.com/sites/default/files/AKR-122-rfp-elisa-kit.pdf"<del class="diffchange diffchange-inline">/ click </del>here<del class="diffchange diffchange-inline">></del></<del class="diffchange diffchange-inline">p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Fig 7. RFP standard curve obtain from the web,<<ins class="diffchange diffchange-inline">a </ins>href="http://www.cellbiolabs.com/sites/default/files/AKR-122-rfp-elisa-kit.pdf"<ins class="diffchange diffchange-inline">>Click </ins>here</<ins class="diffchange diffchange-inline">a</ins>></div></td></tr>
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</table>Sema1688http://2013.igem.org/wiki/index.php?title=Team:HIT-Harbin/Experiments&diff=198954&oldid=prevSema1688 at 06:21, 27 September 20132013-09-27T06:21:10Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Fig 7. RFP standard curve obtain from the web,<del class="diffchange diffchange-inline">[</del>http://www.cellbiolabs.com/sites/default/files/AKR-122-rfp-elisa-kit.pdf click here<del class="diffchange diffchange-inline">]</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Fig 7. RFP standard curve obtain from the web,<ins class="diffchange diffchange-inline"><link href="</ins>http://www.cellbiolabs.com/sites/default/files/AKR-122-rfp-elisa-kit.pdf<ins class="diffchange diffchange-inline">"/ </ins>click here<ins class="diffchange diffchange-inline">></ins></p></div></td></tr>
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