Team:HIT-Harbin/Parts

From 2013.igem.org

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<h5>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</h5>
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<p></p>hrpS gene sequence get from GeneBank
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<p>ATGACGATAAGGAATAGCAATGAATCCGTGCAGTCACTTCATTGTGCCTCCCATGAGTCCTCGTTAACGCATCCGTCGGAAGATATACACGGATCGCTATCTTCTCTCATCCACACTATCGCGCCCCTCAACGTTGATATCGTACTGGAAGGCGAAACCGGCACCGGCAAAGACACGCTGGCGCGCCGTATTCATCAGCTTTCCGGCTGCGCCGGCCCGTTGGTGGCGGTGAACTGCGCCGCCGTGCCGGAAACGCTGGCGGAAAGCGAGTTATTCGGGGTGGTGTCCGGCGCTTACACCGGCGCCAGCCGGTCCCGCGCCGGATACCTTGAAACCGCGGACAAAGGCATTCTGTTTCTGGATGAGATCGACAGTATGCCGCTGACGCTGCAGGCCAAAATGTTACGGGTTCTGGAAAGTCGCGGCATCGAACGGCTGGGCAGCACCCAATTCAAACCGGTGGATATGCGGGTGATTGTCGCCACGCAGACGCCATTGCAGAAACTGGTGGACGAAGGGCGTTTTCGGCGTGACCTCTACTTCCGGCTGGATACGGTAAAAATTCAGCTGCCGACGCTGCGTTCGCGTACGGAAATCATTCTGCCGTTATTCGAGCGCTTTTTGCTCGAGGCCTCGGCCCGCCTGAAACGGCCGGCGCCGGGCCTGTCGGCCATCATTCAGGAACAACTGCTGATGCACGGCTGGCCCGGCAACATTCGTGAGTTGAAGGCGGCGGCCGAGCGCTGGGTGCTGGGGCTGTCGCCGGTGCCGATAGCCAGTGATGGCGACGTGCAGGTAATGGGAGAGGATACGCCGCTGACGTCGCTGAAAGTGCGCCTGCGCCGCATTGAGCGGTTTCTGATTCAGGAAGCGTTGCAGCGCAACGACCACTGTATCGATACGGTGGTGAATGAACTGGGCATTCCCAAACGCACGCTCTATCACCGCATCAAGCTGCTGAATGTGGCGGCGCGCGGGCTGTAATAA</p>
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<p></p>CAI-Value of the sequence before optimization:0.2735861795565913
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<p></p>GC-Content of the sequence before optimization:59.169199594731516
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<p></p>hrpS gene sequence after codon optimization
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<p>ATGACCATCCGTAACTCTAACGAATCTGTTCAGTCTCTGCACTGCGCTTCTCACGAATCTTCTCTGACCCACCCGTCTGAAGACATCCACGGTTCTCTGTCTTCTCTGATCCACACCATCGCTCCGCTGAACGTTGACATCGTTCTGGAAGGTGAAACCGGTACCGGTAAAGACACCCTGGCTCGTCGTATCCACCAGCTGTCTGGTTGCGCTGGTCCGCTGGTTGCTGTTAACTGCGCTGCTGTTCCGGAAACCCTGGCTGAATCTGAACTGTTCGGTGTTGTTTCTGGTGCTTACACCGGTGCTTCTCGTTCTCGTGCTGGTTACCTGGAAACCGCTGACAAAGGTATCCTGTTCCTGGACGAAATCGACTCTATGCCGCTGACCCTGCAAGCTAAAATGCTGCGTGTTCTGGAATCTCGTGGTATCGAACGTCTGGGTTCTACCCAGTTCAAACCGGTTGACATGCGTGTTATCGTTGCTACCCAGACCCCGCTGCAAAAACTGGTTGACGAAGGTCGTTTCCGTCGTGACCTGTACTTCCGTCTGGACACCGTTAAAATCCAGCTGCCGACCCTGCGTTCTCGTACCGAAATCATCCTGCCGCTGTTCGAACGTTTCCTGCTGGAAGCTTCTGCTCGTCTGAAACGTCCGGCTCCGGGTCTGTCTGCTATCATCCAGGAACAGCTGCTGATGCACGGTTGGCCGGGTAACATCCGTGAACTGAAAGCTGCTGCTGAACGTTGGGTTCTGGGTCTGTCTCCGGTTCCGATCGCTTCTGACGGTGACGTTCAGGTTATGGGTGAAGACACCCCGCTGACCTCTCTGAAAGTTCGTCTGCGTCGTATAGAGAGGTTCCTGATCCAGGAGGCTCTGCAACGTAACGACCACTGCATCGACACCGTTGTTAACGAACTGGGTATCCCGAAACGTACCCTGTACCACCGTATCAAACTGCTGAACGTTGCTGCTCGTGGTCTGTAATAA</p>
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<p></p>CAI-Value of the optimized sequence:0.9381699423093808
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<p></p>GC-Content of the optimized sequence:53.596757852077005
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Revision as of 12:41, 26 September 2013

HIT-Harbin

Parts

CODON OPTIMIZATION FOR HRPS AND HRPR GENE

hrpS gene sequence get from GeneBank

ATGACGATAAGGAATAGCAATGAATCCGTGCAGTCACTTCATTGTGCCTCCCATGAGTCCTCGTTAACGCATCCGTCGGAAGATATACACGGATCGCTATCTTCTCTCATCCACACTATCGCGCCCCTCAACGTTGATATCGTACTGGAAGGCGAAACCGGCACCGGCAAAGACACGCTGGCGCGCCGTATTCATCAGCTTTCCGGCTGCGCCGGCCCGTTGGTGGCGGTGAACTGCGCCGCCGTGCCGGAAACGCTGGCGGAAAGCGAGTTATTCGGGGTGGTGTCCGGCGCTTACACCGGCGCCAGCCGGTCCCGCGCCGGATACCTTGAAACCGCGGACAAAGGCATTCTGTTTCTGGATGAGATCGACAGTATGCCGCTGACGCTGCAGGCCAAAATGTTACGGGTTCTGGAAAGTCGCGGCATCGAACGGCTGGGCAGCACCCAATTCAAACCGGTGGATATGCGGGTGATTGTCGCCACGCAGACGCCATTGCAGAAACTGGTGGACGAAGGGCGTTTTCGGCGTGACCTCTACTTCCGGCTGGATACGGTAAAAATTCAGCTGCCGACGCTGCGTTCGCGTACGGAAATCATTCTGCCGTTATTCGAGCGCTTTTTGCTCGAGGCCTCGGCCCGCCTGAAACGGCCGGCGCCGGGCCTGTCGGCCATCATTCAGGAACAACTGCTGATGCACGGCTGGCCCGGCAACATTCGTGAGTTGAAGGCGGCGGCCGAGCGCTGGGTGCTGGGGCTGTCGCCGGTGCCGATAGCCAGTGATGGCGACGTGCAGGTAATGGGAGAGGATACGCCGCTGACGTCGCTGAAAGTGCGCCTGCGCCGCATTGAGCGGTTTCTGATTCAGGAAGCGTTGCAGCGCAACGACCACTGTATCGATACGGTGGTGAATGAACTGGGCATTCCCAAACGCACGCTCTATCACCGCATCAAGCTGCTGAATGTGGCGGCGCGCGGGCTGTAATAA

CAI-Value of the sequence before optimization:0.2735861795565913

GC-Content of the sequence before optimization:59.169199594731516

hrpS gene sequence after codon optimization

ATGACCATCCGTAACTCTAACGAATCTGTTCAGTCTCTGCACTGCGCTTCTCACGAATCTTCTCTGACCCACCCGTCTGAAGACATCCACGGTTCTCTGTCTTCTCTGATCCACACCATCGCTCCGCTGAACGTTGACATCGTTCTGGAAGGTGAAACCGGTACCGGTAAAGACACCCTGGCTCGTCGTATCCACCAGCTGTCTGGTTGCGCTGGTCCGCTGGTTGCTGTTAACTGCGCTGCTGTTCCGGAAACCCTGGCTGAATCTGAACTGTTCGGTGTTGTTTCTGGTGCTTACACCGGTGCTTCTCGTTCTCGTGCTGGTTACCTGGAAACCGCTGACAAAGGTATCCTGTTCCTGGACGAAATCGACTCTATGCCGCTGACCCTGCAAGCTAAAATGCTGCGTGTTCTGGAATCTCGTGGTATCGAACGTCTGGGTTCTACCCAGTTCAAACCGGTTGACATGCGTGTTATCGTTGCTACCCAGACCCCGCTGCAAAAACTGGTTGACGAAGGTCGTTTCCGTCGTGACCTGTACTTCCGTCTGGACACCGTTAAAATCCAGCTGCCGACCCTGCGTTCTCGTACCGAAATCATCCTGCCGCTGTTCGAACGTTTCCTGCTGGAAGCTTCTGCTCGTCTGAAACGTCCGGCTCCGGGTCTGTCTGCTATCATCCAGGAACAGCTGCTGATGCACGGTTGGCCGGGTAACATCCGTGAACTGAAAGCTGCTGCTGAACGTTGGGTTCTGGGTCTGTCTCCGGTTCCGATCGCTTCTGACGGTGACGTTCAGGTTATGGGTGAAGACACCCCGCTGACCTCTCTGAAAGTTCGTCTGCGTCGTATAGAGAGGTTCCTGATCCAGGAGGCTCTGCAACGTAACGACCACTGCATCGACACCGTTGTTAACGAACTGGGTATCCCGAAACGTACCCTGTACCACCGTATCAAACTGCTGAACGTTGCTGCTCGTGGTCTGTAATAA

CAI-Value of the optimized sequence:0.9381699423093808

GC-Content of the optimized sequence:53.596757852077005

<groupparts>iGEM013 HIT-Harbin</groupparts>

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