Team:HIT-Harbin/Parts

From 2013.igem.org

(Difference between revisions)
Line 9: Line 9:
                 </div>
                 </div>
<h5>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</h5>
<h5>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</h5>
-
<p>hrpS gene sequence get from GeneBank</p>
+
<div align="center">
-
<img src="https://2013.igem.org/File:Hrps_sequence_cai_before_optimization.png">
+
<img src="https://2013.igem.org/File:Hrps_sequence_cai_before_optimization.png"/>
 +
<p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p>
 +
</div>
<p></p>CAI-Value of the sequence before optimization:0.2736.    GC-Content of the sequence before optimization:59.17%
<p></p>CAI-Value of the sequence before optimization:0.2736.    GC-Content of the sequence before optimization:59.17%
-
<p></p>hrpS gene sequence after codon optimization
+
<div align="center">
-
 
+
<img src="https://2013.igem.org/File:Hrps_sequence_cai_after_optimization.png"/>
 +
<p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p>
 +
</div>
<p></p>CAI-Value of the optimized sequence:0.9832    GC-Content of the optimized sequence:53.60%
<p></p>CAI-Value of the optimized sequence:0.9832    GC-Content of the optimized sequence:53.60%
-
 
+
<div align="center">
-
<p>hrpR gene sequence get from GeneBank</p>
+
<img src="https://2013.igem.org/File:Hrpr_sequence_cai_before_optimization.png"/>
-
 
+
<p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p>
 +
</div>
<p></p>CAI-Value of the sequence before optimization:0.2490.    GC-Content of the sequence before optimization:57.70
<p></p>CAI-Value of the sequence before optimization:0.2490.    GC-Content of the sequence before optimization:57.70
-
<p></p>hrpR gene sequence after codon optimization
+
<div align="center">
-
 
+
<img src="https://2013.igem.org/File:Hrpr_sequence_cai_after_optimization.png"/>
 +
<p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p>
 +
</div>
<p></p>CAI-Value of the optimized sequence:0.9721.    GC-Content of the optimized sequence:53.69%
<p></p>CAI-Value of the optimized sequence:0.9721.    GC-Content of the optimized sequence:53.69%

Revision as of 13:17, 26 September 2013

HIT-Harbin

Parts

CODON OPTIMIZATION FOR HRPS AND HRPR GENE

Figure1: Codon Adaptation of hrpS gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%

Figure2: Codon Adaptation of hrpS gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%

Figure3: Codon Adaptation of hrpR gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70

Figure4: Codon Adaptation of hrpR gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%

<groupparts>iGEM013 HIT-Harbin</groupparts>