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| - | ==Primer ordered for Module Shuffling Experiments==
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| - | {|class="wikitable"
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| - | ! Identifier !! Order date !! Note !! Sequence
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| - | | IK09:TycA_A1_fw || 2013-07-08 || Colony-PCR of ''Brevibacillus parabrevis'': TycA A domain || ATGTTAGCAAATCAAGCCAATCTC
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| - | | IK10:TycA_A1_rev || 2013-07-08 || Colony-PCR of ''Brevibacillus parabrevis'': TycA A domain || TTGGTTTGCTGTAAGATCAGGCTC
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| - | | IK11:TycB_A1_fw || 2013-07-08 || Colony-PCR of ''Brevibacillus parabrevis'': TycB A1 domain || AATTCGGGAGTCGAGCTTTGTCAG
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| - | | IK12:TycC6_rev || 2013-07-19 || reverse primer for tyrocidine TycC6 module || TTATTTCAGGATGAACAGTTCTTGCAGG
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| - | | IK13:IK13:TycB1-dCom-dC_fw || 2013-07-19 || forward primer for tyrocidine TycB1 module without Com and C domain || GATTGCGTGGCAAACAATTCGGGAGTC
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| - | | IK14:TycB1-TycC6_rev || 2013-07-19 || reverse primer for tyrocidine TycB1 module with Gibson overhang for TycC6 || CAGGCTCGATCGAGAAGACATTCCC TTCCTCCCCGCCTTCCACATACGC
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| - | | IK15:TycC6-TycB1_fw || 2013-07-19 || forward primer for tyrocidine TycC6 module with Gibson overhang for TycB1 || GCGTATGTGGAAGGAGGGGAGGAA GGGAATGTCTTCTCGATCGAGCCTG
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| - | | IK16:TycA_fw || 2013-07-19 || forward primer for tyrocidine TycA module || ATGTTAGCAAATCAGGCCAATCTCATC
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| - | | IK17:TycA-dCom-TycC5_rev || 2013-07-19 || reverse primer for tyrocidine TycA module without Com domain with Gibson overhang for TycC5 || GAATGCGCTCTCGGCAATATGGGC TGTTCGCTCTACTTCTTTTTTCTCGG
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| - | | IK18:TycC5-TycA-dCom_fw || 2013-07-19 || forward primer for tyrocidine TycC5 module with Gibson overhang for TycAdCom || CCGAGAAAAAAGAAGTAGAGCGAACA GCCCATATTGCCGAGAGCGCATTC
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| - | | IK19:TycB1-TycC5_rev || 2013-07-19 || reverse primer for tyrocidine TycB1 module with Gibson overhang for TycC5 || GAATGCGCTCTCGGCAATATGGGC TTCCTCCCCGCCTTCCACATACGC
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| - | | IK20:TycC5-TycB1_fw || 2013-07-19 || forward primer for tyrocidine TycC5 module with Gibson overhang for TycB1 || GCGTATGTGGAAGGAGGGGAGGAA GCCCATATTGCCGAGAGCGCATTC
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| - | | IK21:pSB4K5-TycB1dComdC_rev || 2013-07-19 || reverse primer for mRFP-carrying backbones with Gibson overhang for tyrocidine TycA module (nomenclature wrong) || GTCGATGAGATTGGCCTGATTTGCTAACAT CTAGTATTTCTCCTCTTTCTCTAGTATGTG
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| - | | IK22::pSB4K5-TycC6_fw || 2013-07-19 || forward primer for mRFP-carrying backbones with Gibson overhang for tyrocidine TycC6 module || AACATCCTGCAAGAACTGTTCATCCTGAAA TAATAACGCTGATAGTGCTAGTGTAGATC
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| - | | IK23:pSB4K5-TycA_rev || 2013-07-19 || reverse primer for mRFP-carrying backbones with Gibson overhang for tyrocidine TycB1 module without Com and C domains + start codon (nomenklature wrong) || GACTCCCGAATTGTTTGCCACGCAATCCAT CTAGTATTTCTCCTCTTTCTCTAGTATGTG
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| - | | PW01:TycB_A1_rev || 2013-07-08 || Colony-PCR of ''Brevibacillus parabrevis'': TycB A1 domain || CTTGGCACTTCCTTCAGGCTTC
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| - | | PW02:TycB_E1_fw || 2013-07-08 || Colony-PCR of ''Brevibacillus parabrevis'': TycB E domain || CGAGAGAGCGAGCAAGGTG
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| - | | PW03:TycB_E1_rev || 2013-07-08 || Colony-PCR of ''Brevibacillus parabrevis'': TycB E domain || GTACTCGCCTTCTTCTTTTGC
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| - | | PW04:pSB1C3-TycC5ΔC_rev || 2013-07-19 || Integration of Tetrapeptide NRPS from ''Brevibacillus parabrevis'' in backbone; Gibson primer for Tetrapeptide I & II || TGTTTTGGTTGCGAGGAAGCTGTGCAGCAT CTAGTATTTCTCCTCTTTCTCTAGTATGTG
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| - | | PW05:TycC5ΔC_fwd || 2013-07-19 || Amplification of TycC5-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide I & II || ATGCTGCACAGCTTCCTCGCAACCAAAACAGCC
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| - | | PW06:TycC5ΔC-TycB1ΔCom_rev || 2013-07-19 || Amplification of TycC5-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide I & II || GTGAAACAGCATCCCCTCTTGCATCGG AGGCTCGATCGAGAAGACATTCCCTTTG
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| - | | PW07:TycC5ΔC-TycB1ΔCom_fwd || 2013-07-19 || Amplification of TycB1+C(TycB2)-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide I & II || CAAAGGGAATGTCTTCTCGATCGAGCCT CCGATGCAAGAGGGGATGCTGTTTCAC
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| - | | PW08:C(TycB2)-TycAΔCom_rev || 2013-07-19 || Amplification of TycB1+C(TycB2)-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide I & II || GTTGTCGATGAGATTGGCCTGATTTGCTAACAT GATTTGCGCCAGCTCCTGCTCCGTGTT
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| - | | PW09:C(TycB2)-TycAΔCom_fwd || 2013-07-19 || Amplification of TycA-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide I & II || AACACGGAGCAGGAGCTGGCGCAAATC ATGTTAGCAAATCAGGCCAATCTCATCGACAAC
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| - | | PW10:TycAΔCom-TycC6_rev || 2013-07-19 || Amplification of TycAΔCom-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide I || CTTTGGCTGTCTCTTCGATGAACGC TCGCTCTACTTCTTTTTTCTCGGTGCAATG
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| - | | PW11:TycAΔCom-TycC6_fwd || 2013-07-19 || Amplification of TycC6-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide I || CATTGCACCGAGAAAAAAGAAGTAGAGCGA GCGTTCATCGAAGAGACAGCCAAAG
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| - | | PW12:TycAΔE-TycC6_rev || 2013-07-19 || Amplification of TycAΔE-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide II; one mismatch C->t || CTTTTGCACAGGCTCGATCGAGAAGAC GCTtTTGACAAAAAGAGCAACCTG
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| - | | PW13:TycAΔE-TycC6_fwd || 2013-07-19 || Amplification of TycC6-Module from ''Brevibacillus parabrevis''; Gibson primer for Tetrapeptide II; one mismatch G->a || CAGGTTGCTCTTTTTGTCAAaAGC GTCTTCTCGATCGAGCCTGTGCAAAAG
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