Team:Heidelberg/Templates/DelH week25

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(Created page with "(18.10) ==HM17-FS_78== Annealing temps: 62°C, 60°C, 57°C, 55°C <br/> Elongation time: 2:10 ==Screening remaining Gibson electroporated== 60 colonies were picked and screened ...")
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Revision as of 14:15, 18 October 2013

(18.10)

HM17-FS_78

Annealing temps: 62°C, 60°C, 57°C, 55°C
Elongation time: 2:10

Screening remaining Gibson electroporated

60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62)

Gibson assembly