Team:Heidelberg/Templates/DelH week25

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(Created page with "(18.10) ==HM17-FS_78== Annealing temps: 62°C, 60°C, 57°C, 55°C <br/> Elongation time: 2:10 ==Screening remaining Gibson electroporated== 60 colonies were picked and screened ...")
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60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62)
60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62)
==Gibson assembly==
==Gibson assembly==
 +
{|class="wikitable"
 +
|-
 +
! Reagent !! Volume added (µl)
 +
|-
 +
| BB (FS..) || 0.61
 +
|-
 +
| DN11-FS66 || 1.00
 +
|-
 +
| FS_67-FS_68 || 1.35
 +
|-
 +
| FS_69-FS_70 || 6.34
 +
|-
 +
| FS_71-HM08 || 0.70
 +
|-
 +
| Gibson Master Mix 2x || 10
 +
|}

Revision as of 15:23, 18 October 2013

(18.10)

HM17-FS_78

Annealing temps: 62°C, 60°C, 57°C, 55°C
Elongation time: 2:10

Screening remaining Gibson electroporated

60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62)

Gibson assembly

Reagent Volume added (µl)
BB (FS..) 0.61
DN11-FS66 1.00
FS_67-FS_68 1.35
FS_69-FS_70 6.34
FS_71-HM08 0.70
Gibson Master Mix 2x 10
Retrieved from "http://2013.igem.org/Team:Heidelberg/Templates/DelH_week25"