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Team:Heidelberg/Templates/DelH week26
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==Preparation of large plasmids== | ==Preparation of large plasmids== | ||
6 ml of clones A1, A4, A7, B4 | 6 ml of clones A1, A4, A7, B4 | ||
| + | ==Restriction Digest of Preps with PvuI== | ||
| + | 0.5 µl PvuI-HF, 2 µl CutSmart, 1 µl 1:10 Prep (1-2 µg) <br/> | ||
| + | 2 hours of incubation | ||
| + | ==Screening colonies of pSB4K5+DelH (without promotor)== | ||
| + | 46 colonies <br/> | ||
| + | 0.5 Primer (FS73 and FS95) <br/> | ||
Revision as of 14:18, 21 October 2013
Contents |
21-10-2013
Preparation of large plasmids
6 ml of clones A1, A4, A7, B4
Restriction Digest of Preps with PvuI
0.5 µl PvuI-HF, 2 µl CutSmart, 1 µl 1:10 Prep (1-2 µg)
2 hours of incubation
Screening colonies of pSB4K5+DelH (without promotor)
46 colonies
0.5 Primer (FS73 and FS95)
