Team:Heidelberg/Templates/Del week10 AG

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==05-08-2013==
==05-08-2013==
===Amplification from FS_02 to FS_07; 16.4 kb===
===Amplification from FS_02 to FS_07; 16.4 kb===
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[[File:20130705 DelAG.png|150px|thumb|Gel electrophoresis of DelA-G]]
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[[File:Heidelberg_20130705 DelAG.png|150px|thumb|Gel electrophoresis of DelA-G]]
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! what !! µl   
! what !! µl   
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|-
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| ''D. acidovorans'' || 1
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| ''D. acidovorans'' DSM-39 || 1
|-
|-
| FS_02:  (1/10) || 1
| FS_02:  (1/10) || 1

Latest revision as of 12:24, 30 September 2013

05-08-2013

Amplification from FS_02 to FS_07; 16.4 kb

Gel electrophoresis of DelA-G


Reaction
what µl
D. acidovorans DSM-39 1
FS_02: (1/10) 1
FS_07: (1/10) 1
Phusion Master Mix 10
DMSO 1
dd H2O 6
Conditions
Biorad MyCycler
Cycles-PCR temperature [°C] Time [s]
1 98 10
6 98 1
66 ↓ 0.5 5
72 5min 40s
24 98 1
60 5
72 5min 40s
1 72 17 min
1 4 inf

Results:

  • amplification of DelAG did not work
  • repeat PCR with higher annealing temperature to increase specifity