Team:Heidelberg/Templates/Del week10 AG

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05-08-2013

Amplification from FS_02 to FS_07; 16.4 kb

Gel electrophoresis of DelA-G


Reaction
what µl
D. acidovorans DSM-39 1
FS_02: (1/10) 1
FS_07: (1/10) 1
Phusion Master Mix 10
DMSO 1
dd H2O 6
Conditions
Biorad MyCycler
Cycles-PCR temperature [°C] Time [s]
1 98 10
6 98 1
66 ↓ 0.5 5
72 5min 40s
24 98 1
60 5
72 5min 40s
1 72 17 min
1 4 inf

Results:

  • amplification of DelAG did not work
  • repeat PCR with higher annealing temperature to increase specifity