Team:Heidelberg/Templates/Del week10 pSB4K5

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05-07-2013

Amplification from FS_01 to FS_16; 4.2 kb

Gel electrophoresis of DelF-G, Backbone, DelF-G (5.1 kb), DelG (6.4 kb).
after Gel excision
Reaction
what µl
Template pSB4K5 1
FS_16: (1/10) 1
FS_01: (1/10) 1
Phusion Master Mix 10
dd H2O 6
DMSO 1
Conditions
Biorad MyCycler
Cycles-PCR temperature [°C] Time [s]
1 98 5
14 98 1
62 ↓ 0.5 5
72 3:20 min
16 98 1
60 5
72 3:20 min
1 72 12 min
1 4 inf

Results:

  • Amplification worked well and band was at expected height. Nevertheless the gel band did not run properly on the gel.