http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/Del_week11_AE&feed=atom&action=history
Team:Heidelberg/Templates/Del week11 AE - Revision history
2024-03-29T11:39:56Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/Del_week11_AE&diff=256576&oldid=prev
Nils.kurzawa at 21:38, 30 September 2013
2013-09-30T21:38:59Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:38, 30 September 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==08-07-2013==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==08-07-2013==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Amplification from FS_02 to FS_03; 5.3 kb===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Amplification from FS_02 to FS_03; 5.3 kb===</div></td></tr>
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Nils.kurzawa
http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/Del_week11_AE&diff=251643&oldid=prev
Charly at 01:30, 30 September 2013
2013-09-30T01:30:50Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 01:30, 30 September 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==08-07-2013==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==08-07-2013==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Amplification from FS_02 to FS_03; 5.3 kb===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Amplification from FS_02 to FS_03; 5.3 kb===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:<del class="diffchange diffchange-inline">20130708 </del>DelAE DelBB DelG.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.07) and DelG (07.07); lane1=Marker, lanes2-4=DelAE (5.3kb), lanes5-10=pSB4K5 (4.2kb), lanes11-13:DelG (6.4kb);</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:<ins class="diffchange diffchange-inline">Heidelberg_20130708 </ins>DelAE DelBB DelG.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.07) and DelG (07.07); lane1=Marker, lanes2-4=DelAE (5.3kb), lanes5-10=pSB4K5 (4.2kb), lanes11-13:DelG (6.4kb);</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>run at 100 V, 0.8 % gel (TAE)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>run at 100 V, 0.8 % gel (TAE)]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:'''Reaction'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:'''Reaction'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>! what !! µL </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>! what !! µL </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| ''D. acidovorans'' || 1</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| ''D. acidovorans'' <ins class="diffchange diffchange-inline">DSM-39</ins>|| 1</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| FS_02: (1/10) || 2.5</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| FS_02: (1/10) || 2.5</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:<del class="diffchange diffchange-inline">20130708 </del>DelAE DelBB DelG cut.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.07) and DelG (07.07), cut]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:<ins class="diffchange diffchange-inline">Heidelberg_20130708 </ins>DelAE DelBB DelG cut.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.07) and DelG (07.07), cut]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:'''Conditions'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:'''Conditions'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="wikitable"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| class="wikitable"</div></td></tr>
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Charly
http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/Del_week11_AE&diff=251311&oldid=prev
FloSmi: Created page with "==08-07-2013== ===Amplification from FS_02 to FS_03; 5.3 kb=== [[File:20130708 DelAE DelBB DelG.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.0..."
2013-09-29T22:50:19Z
<p>Created page with "==08-07-2013== ===Amplification from FS_02 to FS_03; 5.3 kb=== [[File:20130708 DelAE DelBB DelG.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.0..."</p>
<p><b>New page</b></p><div>==08-07-2013==<br />
===Amplification from FS_02 to FS_03; 5.3 kb===<br />
[[File:20130708 DelAE DelBB DelG.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.07) and DelG (07.07); lane1=Marker, lanes2-4=DelAE (5.3kb), lanes5-10=pSB4K5 (4.2kb), lanes11-13:DelG (6.4kb);<br />
run at 100 V, 0.8 % gel (TAE)]]<br />
:'''Reaction'''<br />
{| class="wikitable"<br />
|-<br />
! what !! µL <br />
|-<br />
| ''D. acidovorans'' || 1<br />
|-<br />
| FS_02: (1/10) || 2.5<br />
|-<br />
| FS_03: (1/10) || 2.5<br />
|-<br />
| Phusion Master Mix || 25<br />
|-<br />
| DMSO || 2.5<br />
|-<br />
| dd H<sub>2</sub>O || 19<br />
|}<br />
<br />
<br />
[[File:20130708 DelAE DelBB DelG cut.png|150px|thumb|PCR for amplification of del fragments DelAE (08.07), pSB4K5 (08.07) and DelG (07.07), cut]]<br />
:'''Conditions'''<br />
{| class="wikitable"<br />
|-<br />
! colspan="3" | Biorad MyCycler<br />
|-<br />
! Cycles-PCR !! temperature [°C] !! Time [s]<br />
|-<br />
| 1 || 98 || 5<br />
|-<br />
| rowspan="3"| 12 || 98 || 1<br />
|-<br />
| 66 ↓ 0.5 || 5<br />
|-<br />
| 72 || 2:30 min<br />
|-<br />
|rowspan="3"| 18 || 98 || 1<br />
|-<br />
| 63 || 5<br />
|-<br />
| 72 || 2:30 min<br />
|-<br />
| 1 ||72 || 10 min<br />
|-<br />
| 1 || 4 || inf<br />
|}<br />
<br />
'''Results:'''<br />
* Amplification of DelAE worked<br />
* bands were cut out and DNA purified using QIAquick Gel Extraction Kit</div>
FloSmi