Team:Heidelberg/Templates/Del week11 AG

From 2013.igem.org

(Difference between revisions)
(Created page with "==12-07-2013== ===Amplification from FS_02 to FS_07; 16.4 kb=== [[File:20130712 DelAG.png|150px|thumb|PCR for amplification of DelAG (12.07;FS02+FS07); lane6=Ladder log2, lane7=...")
Line 2: Line 2:
===Amplification from FS_02 to FS_07; 16.4 kb===
===Amplification from FS_02 to FS_07; 16.4 kb===
-
[[File:20130712 DelAG.png|150px|thumb|PCR for amplification of DelAG (12.07;FS02+FS07); lane6=Ladder log2, lane7=Ladder 1kb base ruler, lane8=DelAG(12.07;FS02+FS_07) run at 100 V, 0.8 % gel (TAE)]]
+
[[File:Heidelberg_20130712 DelAG.png|150px|thumb|PCR for amplification of DelAG (12.07;FS02+FS07); lane6=Ladder log2, lane7=Ladder 1kb base ruler, lane8=DelAG(12.07;FS02+FS_07) run at 100 V, 0.8 % gel (TAE)]]
:'''Reaction'''
:'''Reaction'''
Line 9: Line 9:
! what !! µl   
! what !! µl   
|-
|-
-
| ''D. acidovorans'' || 1
+
| ''D. acidovorans'' DSM-39 || 1
|-
|-
| FS_02:  (1/10) || 2
| FS_02:  (1/10) || 2

Revision as of 01:52, 30 September 2013

12-07-2013

Amplification from FS_02 to FS_07; 16.4 kb

PCR for amplification of DelAG (12.07;FS02+FS07); lane6=Ladder log2, lane7=Ladder 1kb base ruler, lane8=DelAG(12.07;FS02+FS_07) run at 100 V, 0.8 % gel (TAE)
Reaction
what µl
D. acidovorans DSM-39 1
FS_02: (1/10) 2
FS_07: (1/10) 2
Phusion flash Master Mix 10
DMSO 1
dd H2O 4
Conditions
Biorad MyCycler
Cycles temperature [°C] Time [s]
1 98 10
12 98 1
68 ↓ 0.5 5
72 5:30
18 98 1
66 5
72 5:30
1 72 15min
1 12 inf

Results:

  • only a smear occured, no specific product was amplified
  • PCR will be repeated with a lower, constant annealing temperature