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Team:Heidelberg/Templates/Del week13 E
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(Created page with "==27-07-2013== ===Amplification from DelE (FS_04 to FS_24; 1.6 kb)=== :'''Reaction ''' {| class="wikitable" |- ! what !! µl |- | ''D. acidovorans'' DSM-39|| 1 |- | FS_04: ...") |
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==27-07-2013== | ==27-07-2013== | ||
===Amplification from DelE (FS_04 to FS_24; 1.6 kb)=== | ===Amplification from DelE (FS_04 to FS_24; 1.6 kb)=== | ||
Latest revision as of 13:57, 1 October 2013
27-07-2013
Amplification from DelE (FS_04 to FS_24; 1.6 kb)
- Reaction
| what | µl |
|---|---|
| D. acidovorans DSM-39 | 1 |
| FS_04: (1/10) | 2 |
| FS_24: (1/10) | 2 |
| Phusion flash Master Mix | 10 |
| DMSO | 1 |
| dd H2O | 4 |
- Conditions
| Biometra TProfessional Basic | ||
|---|---|---|
| Cycles | temperature [°C] | Time [s] |
| 1 | 98 | 10 |
| 12 | 98 | 1 |
| 68 ↓ 0.5 | 5 | |
| 72 | 3:00 | |
| 18 | 98 | 1 |
| 66 | 5 | |
| 72 | 3:00 | |
| 1 | 72 | 10 min |
| 1 | 4 | inf |
Results:
- Amplification did not work.
