"
Team:Heidelberg/Templates/Del week14 AE
From 2013.igem.org
(Difference between revisions)
(→Restriction digest of fragment FS_02 to FS_03; 5.3 kb;(27-07-2013; II) with BglII) |
|||
| Line 1: | Line 1: | ||
==29-07-2013== | ==29-07-2013== | ||
| - | ===Restriction digest of fragment FS_02 to FS_03; 5.3 kb;( | + | ===Restriction digest of fragment FS_02 to FS_03; 5.3 kb;(27-07-2013; II) with BglII === |
[[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|100px|thumb|Test restriction digest (29.07); run at 100 V, 0.8 % gel (TAE)]] | [[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|100px|thumb|Test restriction digest (29.07); run at 100 V, 0.8 % gel (TAE)]] | ||
| Line 9: | Line 9: | ||
! what !! µL | ! what !! µL | ||
|- | |- | ||
| - | | FS_02 to FS_03 ( | + | | FS_02 to FS_03 (27-07-2013; II) || 15 |
|- | |- | ||
| BglII || 1 | | BglII || 1 | ||
Revision as of 18:38, 3 October 2013
29-07-2013
Restriction digest of fragment FS_02 to FS_03; 5.3 kb;(27-07-2013; II) with BglII
Incubation at 37°C for about 3 h
| what | µL |
|---|---|
| FS_02 to FS_03 (27-07-2013; II) | 15 |
| BglII | 1 |
| Buffer 3.1 | 2 |
| dd H2O | 2 |
| Expected fragment lengths [bp] | 2146, 1862, 1306 |
Results:
- Restriction digest shows the expected product sizes
- indicator for correct amplicon but to be sure, PCR product will be prepared for single read sequencing by GATC
