"
Team:Heidelberg/Templates/M-25-06-13
From 2013.igem.org
Contents |
DelH Cloning
- DelH colonies grown after 24 h (two big and one small colony)
- No blue colonies
- Anja will replace Fanny and Hannah in the lab until friday
- Suggestion: check on blue colonies only with easy backbone ligation
Indigoidine
- Probably received the right plasmids
- results not as good as in paper (not as blue)
- BAP1 + plasmid is stronger
- Gibson cloning of activating gene and NRPS on one plasmid
- Tomorrow miniprep on picked colonies
- Suggestion: Check expression of ribosome binding site with mCherry-lac system
Genomic integration
- Probably not enough DNA -> PCR with new primers
- Colonies definitely negative
- making fresh competent cells
Software
- Several deadlines for different components
- Deadline 8.7.
- text mining should work
- currently working on components and putting them together
- next meeting on 1.7. 19h at Julia
Miscellaneous
- NRPS estimation of computational feasibility
- Project proposal - lots of feedback -> will be finished tomorrow evening
- NRPS library should maybe get some more space in proposal -> Philipp until tomorrow
- If we lack time in the lab we have to pe prepared properly including all primers.
- We should have an internal meeting before tuesday to discuss about motivation, time we want to spend.
Agenda for next meeting (2.7.2013 19h)
- Report Berlin (Philipp)
- Lab update (all lab people)
- iGEM42 (Julia)
- Motivation & time schedule
- Sponsoring update
Agenda for next meeting (3.7.2013 18h)
- Lab update (all lab people)
