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Team:Heidelberg/Templates/MM week16
From 2013.igem.org
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== 2013-08-12 == | == 2013-08-12 == | ||
| - | [[File: | + | [[File:Heidelberg_2013-08-12 Ilia Sophie Hanna.png|100px|thumb|left|Lane 1: NEB 2-log; lanes 2-4: Colony-PCRs with primers IK01+IK02]] |
* no colonies on 10 µl plate | * no colonies on 10 µl plate | ||
* 2 large colonies, 1 small colony on rest plate | * 2 large colonies, 1 small colony on rest plate | ||
Latest revision as of 02:38, 5 October 2013
2013-08-12
- no colonies on 10 µl plate
- 2 large colonies, 1 small colony on rest plate
- run colony-PCR with primers IK01+IK02:
| Cycles | temperature [°C] | Time [s] |
|---|---|---|
| 1 | 95 | 300 |
| 12 | 95 | 60 |
| 68 ↓0.5°C | 30 | |
| 72 | 120 | |
| 23 | 95 | 60 |
| 62 | 30 | |
| 72 | 120 | |
| 1 | 72 | 600 |
| 1 | 12 | inf |
- genomic integration failed
