http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/MM_week16&feed=atom&action=history
Team:Heidelberg/Templates/MM week16 - Revision history
2024-03-29T09:50:00Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/MM_week16&diff=314836&oldid=prev
Anja R at 02:38, 5 October 2013
2013-10-05T02:38:46Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:38, 5 October 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 2013-08-12 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 2013-08-12 ==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:<del class="diffchange diffchange-inline">2013</del>-08-12 Ilia Sophie Hanna.png|100px|thumb|left|Lane 1: NEB 2-log; lanes 2-4: Colony-PCRs with primers IK01+IK02]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:<ins class="diffchange diffchange-inline">Heidelberg_2013</ins>-08-12 Ilia Sophie Hanna.png|100px|thumb|left|Lane 1: NEB 2-log; lanes 2-4: Colony-PCRs with primers IK01+IK02]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* no colonies on 10 µl plate</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* no colonies on 10 µl plate</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 2 large colonies, 1 small colony on rest plate</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 2 large colonies, 1 small colony on rest plate</div></td></tr>
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Anja R
http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/MM_week16&diff=279219&oldid=prev
Nils.kurzawa at 17:00, 3 October 2013
2013-10-03T17:00:41Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* genomic integration failed</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* genomic integration failed</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">== Experiments planned next: ==</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Category:Lab journal]][[Category:Gold]]</del></div></td><td colspan="2"> </td></tr>
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Nils.kurzawa
http://2013.igem.org/wiki/index.php?title=Team:Heidelberg/Templates/MM_week16&diff=279082&oldid=prev
Nils.kurzawa: Created page with " == 2013-08-12 == Lane 1: NEB 2-log; lanes 2-4: Colony-PCRs with primers IK01+IK02 * no colonies on 10 µl plate * 2 la..."
2013-10-03T16:53:09Z
<p>Created page with " == 2013-08-12 == <a href="/wiki/index.php?title=File:2013-08-12_Ilia_Sophie_Hanna.png&action=edit&redlink=1" class="new" title="File:2013-08-12 Ilia Sophie Hanna.png (page does not exist)">Lane 1: NEB 2-log; lanes 2-4: Colony-PCRs with primers IK01+IK02</a> * no colonies on 10 µl plate * 2 la..."</p>
<p><b>New page</b></p><div><br />
== 2013-08-12 ==<br />
[[File:2013-08-12 Ilia Sophie Hanna.png|100px|thumb|left|Lane 1: NEB 2-log; lanes 2-4: Colony-PCRs with primers IK01+IK02]]<br />
* no colonies on 10 µl plate<br />
* 2 large colonies, 1 small colony on rest plate<br />
* run colony-PCR with primers IK01+IK02:<br />
{| class="wikitable"<br />
|-<br />
! Cycles !! temperature [°C] !! Time [s]<br />
|-<br />
| 1 || 95 || 300<br />
|-<br />
| rowspan="3"| 12 || 95 || 60<br />
|-<br />
| 68 ↓0.5°C || 30<br />
|-<br />
| 72 || 120<br />
|-<br />
| rowspan="3"| 23 || 95 || 60<br />
|-<br />
| 62 || 30<br />
|-<br />
| 72 || 120<br />
|-<br />
| 1 || 72 || 600<br />
|-<br />
| 1 || 12 || inf<br />
|}<br />
* genomic integration failed<br />
<br />
== Experiments planned next: ==<br />
<br />
[[Category:Lab journal]][[Category:Gold]]</div>
Nils.kurzawa