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Team:Heidelberg/Templates/MM week16
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2013-08-12
File:2013-08-12 Ilia Sophie Hanna.png
Lane 1: NEB 2-log; lanes 2-4: Colony-PCRs with primers IK01+IK02
- no colonies on 10 µl plate
- 2 large colonies, 1 small colony on rest plate
- run colony-PCR with primers IK01+IK02:
| Cycles | temperature [°C] | Time [s] |
|---|---|---|
| 1 | 95 | 300 |
| 12 | 95 | 60 |
| 68 ↓0.5°C | 30 | |
| 72 | 120 | |
| 23 | 95 | 60 |
| 62 | 30 | |
| 72 | 120 | |
| 1 | 72 | 600 |
| 1 | 12 | inf |
- genomic integration failed
