Team:Heidelberg/Templates/MM week16
From 2013.igem.org
2013-08-12
- no colonies on 10 µl plate
- 2 large colonies, 1 small colony on rest plate
- run colony-PCR with primers IK01+IK02:
Cycles | temperature [°C] | Time [s] |
---|---|---|
1 | 95 | 300 |
12 | 95 | 60 |
68 ↓0.5°C | 30 | |
72 | 120 | |
23 | 95 | 60 |
62 | 30 | |
72 | 120 | |
1 | 72 | 600 |
1 | 12 | inf |
- genomic integration failed