"
Team:Heidelberg/Templates/MM week16p
From 2013.igem.org
(Difference between revisions)
Nils.kurzawa (Talk | contribs) m (Created page with " == 2013-08-12 == * sequences arrived: File:PIK1.2-pIK2.2 seq 2013-08-12.zip * perform multiple sequence alignment against assumed sequences: [[:File:PIK1.2-2013-08-12 VF2.cl...") |
|||
| Line 1: | Line 1: | ||
| - | |||
== 2013-08-12 == | == 2013-08-12 == | ||
* sequences arrived: [[File:PIK1.2-pIK2.2 seq 2013-08-12.zip]] | * sequences arrived: [[File:PIK1.2-pIK2.2 seq 2013-08-12.zip]] | ||
| - | * perform multiple sequence alignment against assumed sequences: [[:File:PIK1.2-2013-08-12 VF2.clustal|pIK1.2 with VF2]], [[:File:PIK1.2-2013-08-12 VR.clustal|pIK1.2 with VR]], [[:File:PIK2.2-2013-08-12 VF2.clustal|pIK2.2 with VF2]], [[:File:PIK2.2-2013-08-12 VR.clustal|pIK2.2 with VR]] | + | * perform multiple sequence alignment against assumed sequences: [[:File:PIK1.2-2013-08-12 VF2.clustal.txt|pIK1.2 with VF2]], [[:File:PIK1.2-2013-08-12 VR.clustal.txt|pIK1.2 with VR]], [[:File:PIK2.2-2013-08-12 VF2.clustal.txt|pIK2.2 with VF2]], [[:File:PIK2.2-2013-08-12 VR.clustal.txt|pIK2.2 with VR]] |
* pIK1.2 looks good | * pIK1.2 looks good | ||
* part of RBS missing in pIK2.2 -> makes no sense, as part of binding part of IK35, with which the backbone was amplified | * part of RBS missing in pIK2.2 -> makes no sense, as part of binding part of IK35, with which the backbone was amplified | ||
| Line 9: | Line 8: | ||
== 2013-08-13 == | == 2013-08-13 == | ||
* sequences arrived: [[File:PIK1.2-pIK2.6 seq 2013-08-13.zip]] | * sequences arrived: [[File:PIK1.2-pIK2.6 seq 2013-08-13.zip]] | ||
| - | * perform multiple sequence alignment against assumed sequences: [[:File:PIK1.2-2013-08-13 IK25.clustal|pIK1.2 with IK25]], [[:File:PIK2.6-2013-08-13 VF2.clustal|pIK2.6 with VF2]], [[:File:PIK2.6-2013-08-13 VR.clustal|pIK2.6 with VR]] | + | * perform multiple sequence alignment against assumed sequences: [[:File:PIK1.2-2013-08-13 IK25.clustal.txt|pIK1.2 with IK25]], [[:File:PIK2.6-2013-08-13 VF2.clustal.txt|pIK2.6 with VF2]], [[:File:PIK2.6-2013-08-13 VR.clustal.txt|pIK2.6 with VR]] |
* sequences look good | * sequences look good | ||
* [[Preparing glycerol stocks|prepare glycerol stock]] of TOP10-pIK1.2 | * [[Preparing glycerol stocks|prepare glycerol stock]] of TOP10-pIK1.2 | ||
Latest revision as of 23:31, 4 October 2013
2013-08-12
- sequences arrived: File:PIK1.2-pIK2.2 seq 2013-08-12.zip
- perform multiple sequence alignment against assumed sequences: pIK1.2 with VF2, pIK1.2 with VR, pIK2.2 with VF2, pIK2.2 with VR
- pIK1.2 looks good
- part of RBS missing in pIK2.2 -> makes no sense, as part of binding part of IK35, with which the backbone was amplified
- send pIK2.6 to sequencing with primers VF2 and VR, also sequence pIK1.2 with primer IK25 to find out whether RBS is also missing there
2013-08-13
- sequences arrived: File:PIK1.2-pIK2.6 seq 2013-08-13.zip
- perform multiple sequence alignment against assumed sequences: pIK1.2 with IK25, pIK2.6 with VF2, pIK2.6 with VR
- sequences look good
- prepare glycerol stock of TOP10-pIK1.2
2013-08-14
- prepare glycerol stock of TOP10-pIK2.6
