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Team:HokkaidoU Japan/Notebook/Color R0040-S05192-B0015
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| + | <div class="workflow-container"> | ||
| + | <ul class="steps"> | ||
| + | <li class="step start"> | ||
| + | <div class="step-tile"><h2>Start</h2></div> | ||
| + | </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Get chromoprotein DNA</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Aug 19</time> </li> </ul> <div class="step-recipe"> Great thanks to iGEM Uppsala! </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Aug 20</time> </li> </ul> <div class="step-recipe"> S05192<br> 1µL DNA to DH5α </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR & Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Wed Aug 21</time> </li> </ul> <div class="step-recipe"> S05192, B0015<br> We confirmed transformation was completely successful.<br> Cultured them for subsequent experiments. </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> S05192, B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR & PCR purification</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> S05192<br> 100b up, 200b dn, KOD Plus NEO </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> Cut B0015 with EcoRI, XbaI (using 10xM buffer)<br> Cut S05192 with EcoRI, SpeI (using 10xM buffer) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> Ligate B0015 with S05192 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> S05192-B0015<br> 5µL DNA to DH5α </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">ColonyPCR & Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 23</time> </li> </ul> <div class="step-recipe"> S05192-B0015<br> We confirmed transformation was completely successful.<br> Cultured it for subsequent experiments. </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 23</time> </li> </ul> <div class="step-recipe"> R0040<br> 100b up, 200b dn, KOD Plus NEO </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 24</time> </li> </ul> <div class="step-recipe"> S05192-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 24</time> </li> </ul> <div class="step-recipe"> S05192-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 24</time> </li> </ul> <div class="step-recipe"> Ligate S05192-B0015 with R0040 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 24</time> </li> </ul> <div class="step-recipe"> R0040-S05192-B0015<br> 5µL DNA to DH5α </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Aug 26</time> </li> </ul> <div class="step-recipe"> R0040-S05192-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Aug 27</time> </li> </ul> <div class="step-recipe"> R0040-S05192-B0015 </div> </div> </div> </li> | ||
| + | <li class="step complete"> | ||
| + | <div class="step-tile"><h2>Complete!</h2></div> | ||
| + | </li> | ||
| + | </ul> | ||
| + | </div> | ||
<!-- end contents / begin footer --> | <!-- end contents / begin footer --> | ||
Latest revision as of 05:35, 27 September 2013
Maestro E.coli
Notebook
-
Start
-
Get chromoprotein DNA
Great thanks to iGEM Uppsala! -
Transformation
S05192
1µL DNA to DH5α -
Colony PCR & Culture
S05192, B0015
We confirmed transformation was completely successful.
Cultured them for subsequent experiments. -
Mini-prep
S05192, B0015 -
PCR & PCR purification
S05192
100b up, 200b dn, KOD Plus NEO -
Digestion
Cut B0015 with EcoRI, XbaI (using 10xM buffer)
Cut S05192 with EcoRI, SpeI (using 10xM buffer) -
Antarctic phosphatase
B0015 -
Ligation
Ligate B0015 with S05192 -
Transformation
S05192-B0015
5µL DNA to DH5α -
ColonyPCR & Culture
S05192-B0015
We confirmed transformation was completely successful.
Cultured it for subsequent experiments. -
PCR
R0040
100b up, 200b dn, KOD Plus NEO -
Mini-prep
S05192-B0015 -
Antarctic phosphatase
S05192-B0015 -
Ligation
Ligate S05192-B0015 with R0040 -
Transformation
R0040-S05192-B0015
5µL DNA to DH5α -
Culture
R0040-S05192-B0015 -
Mini-prep
R0040-S05192-B0015 -
Complete!
