Team:HokkaidoU Japan/Notebook/RBS SD2,4,6-1K3-Replacement

From 2013.igem.org

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      <h1 id="common-header-title">Maestro E.coli</h1>
 
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      <h2 id="common-header-subtitle">Notebook</h2>
 
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<h2>SD2, 4, 6-1K3 Replacement</h2>
 
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<div class="workflow-container">
 
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  <ul class="steps">
 
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    <li class="step start">
 
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      <div class="step-tile"><h2>Start</h2></div>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 15</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZ&alpha;-B0015 100up 200dn </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 15</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZ&alpha;-B0015 EcoRI and PstI (uning 10&times;H buffer)<br> pSB3K3 vector EcoRI and PstI (using 10&times;H buffer) </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phophatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 16</time> </li> </ul> <div class="step-recipe"> pSB3K3 vector </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 16</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZ&alpha;-B0015 with pSB3K3 </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 17</time> </li> </ul> <div class="step-recipe"> R0040-SD(6/4/2)-LacZ&alpha;-B0015<br> each of 1&micro;L DNA to DH5&alpha; </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Aug 27</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZ&alpha;-B0015 on pSB3K3 </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">Sequence</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Sep 6</time> </li> </ul> <div class="step-recipe"> R-0040-SD(2,4,6,8)-LacZ&alpha;-B0015 on pSB3K3 </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">&beta;-galactosidase assay (1)</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Sep 20</time> </li> </ul> <div class="step-recipe"> R0040-RBS(SD2/4/6/8)-LacZ&alpha;-B0015, X/S (as N. C.) </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">&beta;-galactosidase assay (2)</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Sep 22</time> </li> </ul> <div class="step-recipe"> R0040-RBS(B0034/SD8)-LacZ&alpha;-B0015, X/S (as N. C.) </div> </div> </div> </li>
 
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<li class="step"> <div class="step-tile"> <h2 class="step-title">&beta;-galactosidase assay (3)</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Sep 24</time> </li> </ul> <div class="step-recipe"> R0040-RBS(B0034/SD8)-LacZ&alpha;-B0015, X/S (as N. C.) </div> </div> </div> </li>
 
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    <li class="step complete">
 
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      <div class="step-tile"><h2>Complete!</h2></div>
 
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Latest revision as of 16:58, 27 September 2013

Retrieved from "http://2013.igem.org/Team:HokkaidoU_Japan/Notebook/RBS_SD2,4,6-1K3-Replacement"