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Team:HokkaidoU Japan/Notebook/RBS SD8-1C3-Replacement
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<div class="step-tile"><h2>Start</h2></div> | <div class="step-tile"><h2>Start</h2></div> | ||
</li> | </li> | ||
| - | + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-Prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Mar 23</time> </li> </ul> <div class="step-recipe"> oripro on 1C3 </div> </div> </div> </li> | |
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Mar 28</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015 100up 200dn </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Gel Extraction</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Mar 28</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Mar 28</time> </li> </ul> <div class="step-recipe"> Cut R0040-SD8-LacZα-B0015 on 1A2 and Oripro-B0034-LacZα-B0015 on 1C3 EcoRI and PstI (using 10×H buffer) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Gel Extraction</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Apr 1</time> </li> </ul> <div class="step-recipe"> Oripro-B0034-LacZα-B0015 R0040-SD8-LacZα-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ethanol Precipitation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Apr 2</time> </li> </ul> <div class="step-recipe"> Oripro-B0034-LacZα-B0015 R0040-SD8-LacZα-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Apr 2</time> </li> </ul> <div class="step-recipe"> Ligate R0040-SD8-LacZα-B0015 with Vector(pSB1C3) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Apr 2</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015<br> each of 5µL DNA to DH5α<br><br> faild </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Wed Apr 3</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015<br> each of 5µL DNA to DH5α </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Apr 4</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Apr 6</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Apr 7</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015 </div> </div> </div> </li> | ||
<li class="step complete"> | <li class="step complete"> | ||
Revision as of 16:37, 27 September 2013
Maestro E.coli
Notebook
-
Start
-
Mini-Prep
oripro on 1C3 -
PCR
R0040-SD8-LacZα-B0015 100up 200dn -
Gel Extraction
R0040-SD8-LacZα-B0015 -
Digestion
Cut R0040-SD8-LacZα-B0015 on 1A2 and Oripro-B0034-LacZα-B0015 on 1C3 EcoRI and PstI (using 10×H buffer) -
Gel Extraction
Oripro-B0034-LacZα-B0015 R0040-SD8-LacZα-B0015 -
Ethanol Precipitation
Oripro-B0034-LacZα-B0015 R0040-SD8-LacZα-B0015 -
Ligation
Ligate R0040-SD8-LacZα-B0015 with Vector(pSB1C3) -
Transformation
R0040-SD8-LacZα-B0015
each of 5µL DNA to DH5α
faild -
Transformation
R0040-SD8-LacZα-B0015
each of 5µL DNA to DH5α -
Colony PCR
R0040-SD8-LacZα-B0015 -
Culture
R0040-SD8-LacZα-B0015 -
Mini-prep
R0040-SD8-LacZα-B0015 -
Complete!
