Team:HokkaidoU Japan/Overview

From 2013.igem.org

(Difference between revisions)
 
(14 intermediate revisions not shown)
Line 3: Line 3:
   <div id="common-header-bottom-background">
   <div id="common-header-bottom-background">
     <div class="wrapper">
     <div class="wrapper">
-
       <h1 id="common-header-title">Maestro E.coli</h1>
+
       <h1 id="common-header-title">Maestro <span class="italic">E. coli</span></h1>
       <h2 id="common-header-subtitle">Overview</h2>
       <h2 id="common-header-subtitle">Overview</h2>
       <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png">
       <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png">
Line 10: Line 10:
   <div class="wrapper">
   <div class="wrapper">
-
     <div id="hokkaidou-contents">
+
     <div id="hokkaidou-contents" class="with-arrow-nav">
<!-- end header / begin contents -->
<!-- end header / begin contents -->
Line 25: Line 25:
           Especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable.
           Especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable.
         </p>
         </p>
-
         <p>This year, we  made well-selected sets of promoters and RBSs with dynamic range of strength. We mutated a -35 region of a promoter to make a family of variable promoters. These parts were strictly evaluated. </p>
+
         <p>This year, we  made well-selected sets of promoters and RBSs with dynamic range of strength. We mutated a -35 region of a promoter to make a family of various promoters. And for RBS, we added an enhancer upstream of RBS and change the SD sequence to make a various RBS family. These parts were strictly evaluated. </p>
-
         <p>Furthermore, we make the expresion Optimization Kit which enables you to select the most suitable promoters and RBSs for your project. This can be accomplished in only one step. This Kit will help other iGEMers create more complex and effective devises.</p>
+
         <p>Our project is making the Random Operon Shuffling Kit which enables you to select the most suitable promoters and RBSs for your project. We succeeded in making this kit!
-
         <p>We also did presentation at our university and two high schools about iGEM and genetics. On that occasion we get to know what the people think about gene recombination by means of questionnaires. We tried to provide them with information for deeper thought. We hope these activities will lead the society where people are more accepting genetic engineering. </p>
+
It can be accomplished in only one step. This kit will help other iGEMers create more complex and effective devises.</p>
 +
         <p>We also did presentation at our university and two high schools about iGEM and synthetic biology. On that occasion we get to know what the people think about gene recombination by means of questionnaires. We tried to provide them with information for deeper thought. We hope these activities will lead the society where people are more accepting genetic engineering. </p>
</div>
</div>
 +
 +
<div id="next-page">
 +
<a href="https://2013.igem.org/Team:HokkaidoU_Japan/Overview/Background"><div class="arrow-div"></div><span>Background</span></a>
 +
</div>
 +
 +
<!-- end contents / begin footer -->
<!-- end contents / begin footer -->

Latest revision as of 02:48, 29 October 2013

Maestro E. coli

Overview

Project Description

Recently, more and more projects for iGEM involve precisely regulated systems or multistep catalysis. These biological systems need to have

  1. transparent structure
  2. reliable function
  3. reproducibility
  4. and be safe
Especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable.

This year, we made well-selected sets of promoters and RBSs with dynamic range of strength. We mutated a -35 region of a promoter to make a family of various promoters. And for RBS, we added an enhancer upstream of RBS and change the SD sequence to make a various RBS family. These parts were strictly evaluated.

Our project is making the Random Operon Shuffling Kit which enables you to select the most suitable promoters and RBSs for your project. We succeeded in making this kit! It can be accomplished in only one step. This kit will help other iGEMers create more complex and effective devises.

We also did presentation at our university and two high schools about iGEM and synthetic biology. On that occasion we get to know what the people think about gene recombination by means of questionnaires. We tried to provide them with information for deeper thought. We hope these activities will lead the society where people are more accepting genetic engineering.