http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&feed=atom&action=history
Team:HokkaidoU Japan/RBS/Methods - Revision history
2024-03-29T10:58:19Z
Revision history for this page on the wiki
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http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=359584&oldid=prev
TaKeZo at 02:52, 29 October 2013
2013-10-29T02:52:26Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro E.coli</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro <ins class="diffchange diffchange-inline"><span class="italic"></ins>E. coli<ins class="diffchange diffchange-inline"></span></ins></h1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2 id="common-header-subtitle">RBS</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2 id="common-header-subtitle">RBS</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png"></div></td></tr>
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TaKeZo
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=354046&oldid=prev
Kwt at 23:23, 28 October 2013
2013-10-28T23:23:02Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> We constructed new RBS family, SD2, SD4, SD6, SD8.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> We constructed new RBS family, SD2, SD4, SD6, SD8.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> These RBSs have <del class="diffchange diffchange-inline">Enhancer </del>sequence (GCTCTTTAACAATTTATCA) and SD sequence (SD2:GG, SD4:GAGG, SD6:AGGAGG, SD8:TAAGGAGG).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> These RBSs have <ins class="diffchange diffchange-inline">enhancer </ins>sequence (GCTCTTTAACAATTTATCA) and SD sequence (SD2:GG, SD4:GAGG, SD6:AGGAGG, SD8:TAAGGAGG).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> We constructed SD8 from synthetic oligos (forward:SD8-f, reverse:SD8-r).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> We constructed SD8 from synthetic oligos (forward:SD8-f, reverse:SD8-r).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> We constructed SD2, SD4, SD6 by PCR (forward:EX-f, reverse:SD2-r, SD4-r, SD6-r, template:SD8).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> We constructed SD2, SD4, SD6 by PCR (forward:EX-f, reverse:SD2-r, SD4-r, SD6-r, template:SD8).</div></td></tr>
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Kwt
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=335252&oldid=prev
Nousan at 06:43, 27 October 2013
2013-10-27T06:43:24Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig fig400 para"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig fig400 para"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div><span class="bold">fig.2: RBS construction</span></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div><span class="bold">fig.2: RBS construction<ins class="diffchange diffchange-inline">.</ins></span></div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/1/1a/HokkaidoU2013_RBS_methods3revision_400.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/1/1a/HokkaidoU2013_RBS_methods3revision_400.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div><span class="bold">fig.3: our parts</span></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div><span class="bold">fig.3: our parts<ins class="diffchange diffchange-inline">.</ins></span></div></div></td></tr>
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Nousan
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=335250&oldid=prev
Nousan at 06:43, 27 October 2013
2013-10-27T06:43:08Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig fig800"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig fig800"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/a/ab/HokkaidoU_RBS_methods1_800.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/a/ab/HokkaidoU_RBS_methods1_800.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div>fig.1: oligos<del class="diffchange diffchange-inline">; </del>RED: enhancer sequence, BLUE: SD sequence.</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div<ins class="diffchange diffchange-inline">><span class="bold"</ins>>fig.1: oligos<ins class="diffchange diffchange-inline">.</span> </ins>RED: enhancer sequence, BLUE: SD sequence.</div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig fig400 para"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig fig400 para"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div>fig.2: RBS construction</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div<ins class="diffchange diffchange-inline">><span class="bold"</ins>>fig.2: RBS construction<ins class="diffchange diffchange-inline"></span></ins></div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/1/1a/HokkaidoU2013_RBS_methods3revision_400.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/1/1a/HokkaidoU2013_RBS_methods3revision_400.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div>fig.3: our parts</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div<ins class="diffchange diffchange-inline">><span class="bold"</ins>>fig.3: our parts<ins class="diffchange diffchange-inline"></span></ins></div></div></td></tr>
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Nousan
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=326327&oldid=prev
Nousan at 08:16, 24 October 2013
2013-10-24T08:16:59Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Assay</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Assay</h2></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> We ligated TetR repressible promoter (<del class="diffchange diffchange-inline">pTET</del>), each of the new RBSs', LacZ&alpha; and double terminator.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> We ligated TetR repressible promoter (<ins class="diffchange diffchange-inline">pTet</ins>), each of the new RBSs', LacZ&alpha; and double terminator.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Using this construct we performed &beta;-Galactosidase assay.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Using this construct we performed &beta;-Galactosidase assay.</div></td></tr>
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Nousan
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=324327&oldid=prev
Barao at 11:51, 22 October 2013
2013-10-22T11:51:26Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Assay</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Assay</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> We ligated TetR repressible promoter (<del class="diffchange diffchange-inline">pTet</del>), each of the new RBSs', LacZ&alpha; and double terminator.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> We ligated TetR repressible promoter (<ins class="diffchange diffchange-inline">pTET</ins>), each of the new RBSs', LacZ&alpha; and double terminator.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Using this construct we performed &beta;-Galactosidase assay.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Using this construct we performed &beta;-Galactosidase assay.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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Barao
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=322543&oldid=prev
Barao at 14:53, 20 October 2013
2013-10-20T14:53:32Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/<del class="diffchange diffchange-inline">2</del>/<del class="diffchange diffchange-inline">28</del>/<del class="diffchange diffchange-inline">HokkaidoU_RBS_methods3_400</del>.png"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/<ins class="diffchange diffchange-inline">1</ins>/<ins class="diffchange diffchange-inline">1a</ins>/<ins class="diffchange diffchange-inline">HokkaidoU2013_RBS_methods3revision_400</ins>.png"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.3: our parts</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.3: our parts</div></div></td></tr>
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Barao
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=230392&oldid=prev
TaKeZo at 00:36, 28 September 2013
2013-09-28T00:36:08Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/a/ab/HokkaidoU_RBS_methods1_800.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/a/ab/HokkaidoU_RBS_methods1_800.png"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.1: oligos; RED: enhancer sequence, BLUE: SD sequence.</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.1: oligos; RED: enhancer sequence, BLUE: SD sequence.</div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="fig400 para"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="<ins class="diffchange diffchange-inline">fig </ins>fig400 para"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.2: RBS construction</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.2: RBS construction</div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.3: our parts</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div>fig.3: our parts</div></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="prev-page"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="https://2013.igem.org/Team:HokkaidoU_Japan/RBS"><div class="arrow-div"></div><span>RBS Top</span></a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="next-page"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="https://2013.igem.org/Team:HokkaidoU_Japan/RBS/Results"><div class="arrow-div"></div><span>Results</span></a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
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TaKeZo
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=227962&oldid=prev
Nousan at 23:45, 27 September 2013
2013-09-27T23:45:26Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:45, 27 September 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig800"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig800"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/a/ab/HokkaidoU_RBS_methods1_800.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/a/ab/HokkaidoU_RBS_methods1_800.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div><del class="diffchange diffchange-inline">Fig</del>.1: oligos; RED: enhancer sequence, BLUE: SD sequence.</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div><ins class="diffchange diffchange-inline">fig</ins>.1: oligos; RED: enhancer sequence, BLUE: SD sequence.</div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig400 para"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="fig400 para"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/9/9e/HokkaidoU_RBS_methods2_400.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div><del class="diffchange diffchange-inline">Fig</del>.2: RBS construction</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div><ins class="diffchange diffchange-inline">fig</ins>.2: RBS construction</div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/2/28/HokkaidoU_RBS_methods3_400.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013/2/28/HokkaidoU_RBS_methods3_400.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div><del class="diffchange diffchange-inline">Fig</del>.3: our parts</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div><ins class="diffchange diffchange-inline">fig</ins>.3: our parts</div></div></td></tr>
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Nousan
http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/RBS/Methods&diff=223277&oldid=prev
Nousan at 21:50, 27 September 2013
2013-09-27T21:50:38Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Assay</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Assay</h2></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> We ligated TetR repressible promoter (<del class="diffchange diffchange-inline">pTET</del>), each of the new RBSs', LacZ&alpha; and double terminator.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> We ligated TetR repressible promoter (<ins class="diffchange diffchange-inline">pTet</ins>), each of the new RBSs', LacZ&alpha; and double terminator.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Using this construct we performed &beta;-Galactosidase assay.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Using this construct we performed &beta;-Galactosidase assay.</div></td></tr>
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Nousan