http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&feed=atom&action=historyTeam:HokkaidoU Japan/Shuffling Kit/Future Work - Revision history2024-03-29T08:13:16ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=359516&oldid=prevTaKeZo at 02:50, 29 October 20132013-10-29T02:50:46Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro E. coli</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro <ins class="diffchange diffchange-inline"><span class="italic"></ins>E. coli<ins class="diffchange diffchange-inline"></span></ins></h1></div></td></tr>
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</table>TaKeZohttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=359057&oldid=prevBarao at 02:36, 29 October 20132013-10-29T02:36:33Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h1>Maestro E.coli Future Work</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h1>Maestro <ins class="diffchange diffchange-inline"><span class="italic"></ins>E. coli<ins class="diffchange diffchange-inline"></span> </ins>Future Work</h1></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td></tr>
</table>Baraohttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=358849&oldid=prevBarao at 02:30, 29 October 20132013-10-29T02:30:45Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro E.coli</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro E. coli</h1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2 id="common-header-subtitle">Shuffling Kit</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2 id="common-header-subtitle">Shuffling Kit</h2></div></td></tr>
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</table>Baraohttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=357841&oldid=prevKwt at 01:53, 29 October 20132013-10-29T01:53:54Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>To make Promoter Selector better, we are going to place pigment producing construct between two PstI sites. After you confirm the best promoter, you can remove the <del class="diffchange diffchange-inline">latter </del>constructs by digesting with PstI.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>To make Promoter Selector better, we are going to place pigment producing construct between two PstI sites. After you confirm the best promoter, you can remove the <ins class="diffchange diffchange-inline">followed </ins>constructs by digesting with PstI.</p></div></td></tr>
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</table>Kwthttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=357785&oldid=prevKwt at 01:50, 29 October 20132013-10-29T01:50:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>To make Promoter Selector better, we are going to place pigment producing construct between two PstI sites. After you confirm the best promoter, you can remove <del class="diffchange diffchange-inline">pigment producing </del>constructs by digesting with PstI.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>To make Promoter Selector better, we are going to place pigment producing construct between two PstI sites. After you confirm the best promoter, you can remove <ins class="diffchange diffchange-inline">the latter </ins>constructs by digesting with PstI.</p></div></td></tr>
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</table>Kwthttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=350879&oldid=prevBarao at 19:57, 28 October 20132013-10-28T19:57:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro E.coli</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h1 id="common-header-title">Maestro E.coli</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h2 id="common-header-subtitle"><del class="diffchange diffchange-inline">Optimization </del>Kit</h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h2 id="common-header-subtitle"><ins class="diffchange diffchange-inline">Shuffling </ins>Kit</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>Baraohttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=350499&oldid=prevAmigo at 19:35, 28 October 20132013-10-28T19:35:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>To make Promoter Selector better kit</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>To make Promoter Selector better, we are going to <del class="diffchange diffchange-inline">put </del>pigment producing <del class="diffchange diffchange-inline">constructs </del>between two PstI sites. After you confirm promoter, you can remove pigment producing constructs by PstI.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>To make Promoter Selector better, we are going to <ins class="diffchange diffchange-inline">place </ins>pigment producing <ins class="diffchange diffchange-inline">construct </ins>between two PstI sites. After you confirm <ins class="diffchange diffchange-inline">the best </ins>promoter, you can remove pigment producing constructs by <ins class="diffchange diffchange-inline">digesting with </ins>PstI.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We can get a lot of operons by RBS Selector. RBS Selector provides 16 operons in case of 2 CDS, 64 operons in case of 3 CDS. What can we do using these number of operons? We expect industrial applications of RBS Selector as future works.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We can get a lot of operons by RBS Selector. RBS Selector provides 16 operons in case of 2 CDS, 64 operons in case of 3 CDS. What can we do using these number of operons? We expect industrial applications of RBS Selector as future works.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>For example PHB </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>(polyhydroxybutyrate) ,3 enzymes synthesize PHB from substrate.PHB is one of biodegradable plastic. You can learn more about PHB at <a href="https://2012.igem.org/Team:HokkaidoU_Japan/Project/PHB_Synthesis">iGEM HokkaidoU_Japan 2012 wiki</a>. <del class="diffchange diffchange-inline"> However, you know by our wiki</del>, CDS has <del class="diffchange diffchange-inline">each </del>optimum RBS. <del class="diffchange diffchange-inline">It is possibility to find more efficient construct from 64 constructs by assay results</del>. It is possible to use RBS Selector <del class="diffchange diffchange-inline">to </del>medicine or vaccine etc.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In the case of producing valuable molecules inside the cell, it is important to select the RBS which results the largest yield.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We hope applications of our project like making world happy.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For example PHB </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>(polyhydroxybutyrate) ,3 enzymes synthesize PHB from substrate. PHB is one of <ins class="diffchange diffchange-inline">the eco-friendly, </ins>biodegradable plastic. You can learn more about PHB at <a href="https://2012.igem.org/Team:HokkaidoU_Japan/Project/PHB_Synthesis">iGEM HokkaidoU_Japan 2012 wiki</a>. <ins class="diffchange diffchange-inline">In the production of PHB</ins>, <ins class="diffchange diffchange-inline">each </ins>CDS has <ins class="diffchange diffchange-inline">the </ins>optimum RBS. <ins class="diffchange diffchange-inline">Our RS can be used for selecting the best combination of RBSs</ins>.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>It is possible to use RBS Selector <ins class="diffchange diffchange-inline">when producing </ins>medicine or vaccine etc.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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</table>Amigohttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=349589&oldid=prevNousan at 18:25, 28 October 20132013-10-28T18:25:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Application of RBS Selector</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Application of RBS Selector</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We can get a lot of operons by RBS Selector. RBS Selector provides 16 operons in 2 CDS <del class="diffchange diffchange-inline">case</del>, 64 operons in 3 CDS <del class="diffchange diffchange-inline">case</del>. What can we do using these number of operons? We expect industrial applications of RBS Selector as future works.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>We can get a lot of operons by RBS Selector. RBS Selector provides 16 operons in <ins class="diffchange diffchange-inline">case of </ins>2 CDS, 64 operons in <ins class="diffchange diffchange-inline">case of </ins>3 CDS. What can we do using these number of operons? We expect industrial applications of RBS Selector as future works.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example PHB </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example PHB </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>(polyhydroxybutyrate) , <del class="diffchange diffchange-inline">you </del>can learn more about PHB at <a href="https://2012.igem.org/Team:HokkaidoU_Japan/Project/PHB_Synthesis">iGEM HokkaidoU_Japan 2012 wiki</a>. <del class="diffchange diffchange-inline">PHB is one of biodegradable plastic. 3 enzymes synthesize PHB from substrate. </del>However, you know by our wiki, CDS has each optimum RBS. It is possibility to find more efficient construct from 64 constructs by assay results. It is possible to use RBS Selector to medicine or vaccine etc.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>(polyhydroxybutyrate) ,<ins class="diffchange diffchange-inline">3 enzymes synthesize PHB from substrate.PHB is one of biodegradable plastic. You </ins>can learn more about PHB at <a href="https://2012.igem.org/Team:HokkaidoU_Japan/Project/PHB_Synthesis">iGEM HokkaidoU_Japan 2012 wiki</a>. <ins class="diffchange diffchange-inline"> </ins>However, you know by our wiki, CDS has each optimum RBS. It is possibility to find more efficient construct from 64 constructs by assay results. It is possible to use RBS Selector to medicine or vaccine etc.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We hope applications of our project like making world happy.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We hope applications of our project like making world happy.</div></td></tr>
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</table>Nousanhttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=347251&oldid=prevTaKeZo at 15:27, 28 October 20132013-10-28T15:27:30Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="https://2013.igem.org/Team:HokkaidoU_Japan/<del class="diffchange diffchange-inline">Optimization</del>/Examples"><div class="arrow-div"></div><span>Examples</span></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><a href="https://2013.igem.org/Team:HokkaidoU_Japan/<ins class="diffchange diffchange-inline">Shuffling_Kit</ins>/Examples"><div class="arrow-div"></div><span>Examples</span></a></div></td></tr>
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</table>TaKeZohttp://2013.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work&diff=346939&oldid=prevTaKeZo: moved Team:HokkaidoU Japan/Optimization/Future Work to Team:HokkaidoU Japan/Shuffling Kit/Future Work2013-10-28T15:03:16Z<p>moved <a href="/Team:HokkaidoU_Japan/Optimization/Future_Work" class="mw-redirect" title="Team:HokkaidoU Japan/Optimization/Future Work">Team:HokkaidoU Japan/Optimization/Future Work</a> to <a href="/Team:HokkaidoU_Japan/Shuffling_Kit/Future_Work" title="Team:HokkaidoU Japan/Shuffling Kit/Future Work">Team:HokkaidoU Japan/Shuffling Kit/Future Work</a></p>
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