Team:HokkaidoU Japan/Shuffling Kit/How To Use

From 2013.igem.org

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<h2>How to use</h2>
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<h1>How to use</h1>
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<h3>What users should prepare</h3>
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<h2>What users should prepare</h2>
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   To use the kit, the protein sequence you chose must have a BsaI site and an which will produce overhang. Therefore, users must design a primer to add it. To reduce time and trouble, we automated the design by creating program <a href="https://2013.igem.org/Team:HokkaidoU_Japan/Optimization/Primer_Designer">"POK-ROK Primer Designer"!</a>
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   To use the kit, the protein sequence you chose must have specific prefix/suffix which contains a BsaI site and produce overhang. Therefore, users must design a primer to add it. To reduce time and trouble, we automated the design by creating program <a href="https://2013.igem.org/Team:HokkaidoU_Japan/Optimization/Primer_Designer">"POK-ROK Primer Designer"!</a>
</p>
</p>

Revision as of 15:12, 27 September 2013

Maestro E.coli

Optimization Kit

How to use

What users should prepare

To use the kit, the protein sequence you chose must have specific prefix/suffix which contains a BsaI site and produce overhang. Therefore, users must design a primer to add it. To reduce time and trouble, we automated the design by creating program "POK-ROK Primer Designer"!