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Team:HokkaidoU Japan/Shuffling Kit/Primer Designer
From 2013.igem.org
(Difference between revisions)
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<html> | <html> | ||
| + | <body> | ||
<form id="primer-designer"> | <form id="primer-designer"> | ||
Overhang_F <input type="text" name="overhang-f"><br> | Overhang_F <input type="text" name="overhang-f"><br> | ||
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(function(){var f,g,e,d,c,b,a;e=function(h){switch(h){case"AA":case"TT":return -9.1;case"AT":return -8.6;case"TA":return -6;case"CA":case"TG":return -5.8;case"GT":case"AC":return -6.5;case"CT":case"AG":return -7.8;case"GA":case"TC":return -5.6;case"CG":return -11.9;case"GC":return -11.1;case"GG":case"CC":return -11}};b=function(h){var j,k,m,l;k=0;for(j=m=0,l=h.length-2;0<=l?m<=l:m>=l;j=0<=l?++m:--m){k+=e(h.slice(j,+(j+1)+1||9000000000))}return k};d=function(h){switch(h){case"AA":case"TT":return -24;case"AT":return -23.9;case"TA":return -16.9;case"CA":case"TG":return -12.9;case"GT":case"AC":return -17.3;case"CT":case"AG":return -20.8;case"GA":case"TC":return -13.5;case"CG":return -27.8;case"GC":return -26.7;case"GG":case"CC":return -26.6}};a=function(h){var j,k,m,l;k=0;for(j=m=0,l=h.length-2;0<=l?m<=l:m>=l;j=0<=l?++m:--m){k+=d(h.slice(j,+(j+1)+1||9000000000))}return k};f=function(i){var k,j;k=b(i);j=a(i);return(1000*k/(-10.8+j+1.987*-15.89495209964411))-273.15+16.6*-1.3010299956639813};g=function(i){var k,h,j;if(f(i.slice(0,35))<60){alert("Sequence is too short.")}for(k=j=17;j<=35;k=++j){h=f(i.slice(0,+(k-1)+1||9000000000));if(h>60&&(i[k-1]==="G"||i[k-1]==="C")){break}}return[i.slice(0,+(k-1)+1||9000000000),h]};c=function(l,h,p){var m,i,k,n,o,j;m=g(p);i=g(p.split("").reverse().join(""));k="TTTGGTCTCT"+l+"T"+m[0];o=m[1];n="TTTGGTCTCA"+l+"A"+i[0];j=i[1];return[k,o,n,j]};$(function(){return $("#primer-designer").submit(function(k){var j,i,n,l,h,m;k.preventDefault();i=$(this);j=i.find("button");j.attr("disabled",true);n=i.find('[name="overhang-f"]').val().toUpperCase();l=i.find('[name="overhang-r"]').val().toUpperCase();m=i.find('[name="sequence"]').val().toUpperCase();h=c(n,l,m);$("#primer-f").text(h[0]);$("#tm-f").text(h[1]);$("#primer-r").text(h[2]);$("#tm-r").text(h[3]);return j.attr("disabled",false)})})}).call(this); | (function(){var f,g,e,d,c,b,a;e=function(h){switch(h){case"AA":case"TT":return -9.1;case"AT":return -8.6;case"TA":return -6;case"CA":case"TG":return -5.8;case"GT":case"AC":return -6.5;case"CT":case"AG":return -7.8;case"GA":case"TC":return -5.6;case"CG":return -11.9;case"GC":return -11.1;case"GG":case"CC":return -11}};b=function(h){var j,k,m,l;k=0;for(j=m=0,l=h.length-2;0<=l?m<=l:m>=l;j=0<=l?++m:--m){k+=e(h.slice(j,+(j+1)+1||9000000000))}return k};d=function(h){switch(h){case"AA":case"TT":return -24;case"AT":return -23.9;case"TA":return -16.9;case"CA":case"TG":return -12.9;case"GT":case"AC":return -17.3;case"CT":case"AG":return -20.8;case"GA":case"TC":return -13.5;case"CG":return -27.8;case"GC":return -26.7;case"GG":case"CC":return -26.6}};a=function(h){var j,k,m,l;k=0;for(j=m=0,l=h.length-2;0<=l?m<=l:m>=l;j=0<=l?++m:--m){k+=d(h.slice(j,+(j+1)+1||9000000000))}return k};f=function(i){var k,j;k=b(i);j=a(i);return(1000*k/(-10.8+j+1.987*-15.89495209964411))-273.15+16.6*-1.3010299956639813};g=function(i){var k,h,j;if(f(i.slice(0,35))<60){alert("Sequence is too short.")}for(k=j=17;j<=35;k=++j){h=f(i.slice(0,+(k-1)+1||9000000000));if(h>60&&(i[k-1]==="G"||i[k-1]==="C")){break}}return[i.slice(0,+(k-1)+1||9000000000),h]};c=function(l,h,p){var m,i,k,n,o,j;m=g(p);i=g(p.split("").reverse().join(""));k="TTTGGTCTCT"+l+"T"+m[0];o=m[1];n="TTTGGTCTCA"+l+"A"+i[0];j=i[1];return[k,o,n,j]};$(function(){return $("#primer-designer").submit(function(k){var j,i,n,l,h,m;k.preventDefault();i=$(this);j=i.find("button");j.attr("disabled",true);n=i.find('[name="overhang-f"]').val().toUpperCase();l=i.find('[name="overhang-r"]').val().toUpperCase();m=i.find('[name="sequence"]').val().toUpperCase();h=c(n,l,m);$("#primer-f").text(h[0]);$("#tm-f").text(h[1]);$("#primer-r").text(h[2]);$("#tm-r").text(h[3]);return j.attr("disabled",false)})})}).call(this); | ||
</script> | </script> | ||
| + | |||
| + | </body> | ||
</html> | </html> | ||
Revision as of 12:09, 19 September 2013
Forward
- sequence
- tm
Reverse
- sequence
- tm
