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Team:HokkaidoU Japan/Shuffling Kit/Primer Designer
From 2013.igem.org
(Difference between revisions)
| Line 124: | Line 124: | ||
for (n = _i = 17; _i <= 35; n = ++_i) { | for (n = _i = 17; _i <= 35; n = ++_i) { | ||
tm = calculate_tm(seq.slice(0, +(n - 1) + 1 || 9e9)); | tm = calculate_tm(seq.slice(0, +(n - 1) + 1 || 9e9)); | ||
| - | if (tm > 60 | + | if (tm > 60) { |
| - | + | if (seq[n - 1] === 'G' || seq[n - 1] === 'C') { | |
| + | break; | ||
| + | } | ||
} | } | ||
} | } | ||
Revision as of 12:18, 19 September 2013
Forward
- sequence
- tm
Reverse
- sequence
- tm
