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Team:HokkaidoU Japan/Shuffling Kit/Primer Designer
From 2013.igem.org
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<!-- end header / begin contents --> | <!-- end header / begin contents --> | ||
Revision as of 13:17, 27 September 2013
Maestro E.coli
Optimization Kit
GGA Primer Designer
1. First, you have to decide whether or not you conform to our default overhang set.
2. Secondly, you hove to determine how many CDS are included in your target plasmid.
I have CDS(s) included.3. Then, please select the region to where your biobrick correspond in target plasmid.
1
2
3
8
9
4. Okey, now we are ready to go. Enter your biobrick's sequence, and press "Calculate"!
2. Please enter overhangs and biobrick's sequence, and press "Calculate"
Forward
- sequence
- tm
Reverse
- sequence
- tm
