Team:HokkaidoU Japan/Shuffling Kit/Primer Designer

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             <h3>5. Now, repeat previous step for remaining biobricks included in the target plasmid.</h3>
             <h3>5. Now, repeat previous step for remaining biobricks included in the target plasmid.</h3>
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Revision as of 14:11, 27 September 2013

Maestro E.coli

Optimization Kit

GGA Primer Designer

1. First, you have to decide whether or not you conform to our default overhang set.

2. Secondly, you hove to determine how many CDS are included in your target plasmid.

I have CDS(s) included.

3. Then, please select the region to where your biobrick correspond in target plasmid.

1
2
3
4
5
6
7
8
9

4. Okey, now we are ready to go. Enter your biobrick's sequence, and press "Calculate"!

2. Please enter overhangs and biobrick's sequence, and press "Calculate"





Forward

sequence
tm

Reverse

sequence
tm

5. Now, repeat previous step for remaining biobricks included in the target plasmid.