Team:HokkaidoU Japan/Shuffling Kit/Primer Designer

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Revision as of 00:28, 28 September 2013 by TaKeZo (Talk | contribs)

Maestro E.coli

Optimization Kit

POK-ROK Primer Designer

1. First, you have to decide whether or not you conform to our default overhang set.

2. Secondly, you hove to determine how many CDS are included in your target plasmid.

I have CDS(s) included.

3. Then, please select the region to where your flagment correspond in target plasmid.

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4. Okey, now we are ready to go. Enter your flagment's sequence, and press "Calculate"!

2. Please enter overhangs and flagment's sequence, and press "Calculate"





Forward

sequence
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Reverse

sequence
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5. Now, repeat previous step for remaining flagments included in the target plasmid.