Team:Hong Kong CUHK/backgroundVS

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     <p>Voltage Switch is a response system triggered by a change of potential  across the bacterial inner membrane. Components of this system include the transmembrane  voltage sensor peptide originated from potassium ion channels, polyproline  linker in the inner membrane, glycine serine linker, PDZ domain and PDZ ligand  in the periplasm, and glycine serine linker and effectors in the cytosol.</p>
     <p>Voltage Switch is a response system triggered by a change of potential  across the bacterial inner membrane. Components of this system include the transmembrane  voltage sensor peptide originated from potassium ion channels, polyproline  linker in the inner membrane, glycine serine linker, PDZ domain and PDZ ligand  in the periplasm, and glycine serine linker and effectors in the cytosol.</p>
<h3>1. Components of Voltage Switch System</h3><p align="center">
<h3>1. Components of Voltage Switch System</h3><p align="center">
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   <img width="36" height="36" src="file:///C|/Users/Janecube/AppData/Roaming/Adobe/Dreamweaver CS6/zh_TW/OfficeImageTemp/clip_image001.png" alt="1"><img src="https://static.igem.org/mediawiki/2013/8/84/Vs1.png" alt="2" width="521" height="270"> <br>
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   <img src="https://static.igem.org/mediawiki/2013/8/84/Vs1.png" alt="2" width="521" height="270"> <br>
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<p><stong>
<p><stong>
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   These effectors can be changed accordingly. In our project, we chose  laccase and dioxygenase for BaP degradation. We also planned to test the system  with bimolecular fluorescence complementation (BiFC) dimerization.  
   These effectors can be changed accordingly. In our project, we chose  laccase and dioxygenase for BaP degradation. We also planned to test the system  with bimolecular fluorescence complementation (BiFC) dimerization.  
To become functional, the PDZ domain and PDZ ligand bind the two protein  fragments together, forming a stable dimer.</p>
To become functional, the PDZ domain and PDZ ligand bind the two protein  fragments together, forming a stable dimer.</p>
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<p><h3>2. Proposed Mechanism of Switching &ldquo;ON&rdquo; and &ldquo;OFF&rdquo; in the System<h3></p>
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<p><h3>2. Proposed Mechanism of Switching &ldquo;ON&rdquo; and &ldquo;OFF&rdquo; in the System</h3></p>
<p><strong>Switching &ldquo;OFF&rdquo;</strong><br>
<p><strong>Switching &ldquo;OFF&rdquo;</strong><br>
   When no external charge is applied, the two effectors are combined, as  the repulsion force between the two positively-charged voltage sensor peptide  is relatively smaller than the dimer effect. The two voltage sensor peptides  are vertically parallel to each other.</p>
   When no external charge is applied, the two effectors are combined, as  the repulsion force between the two positively-charged voltage sensor peptide  is relatively smaller than the dimer effect. The two voltage sensor peptides  are vertically parallel to each other.</p>

Latest revision as of 03:57, 29 October 2013

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