Team:Hong Kong CUHK/pah
From 2013.igem.org
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- | <p2> < | + | <p2> <h3>T7-RBS-QsrR (Device <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092001">BBa_K1092001</a>)</h3> |
<p>Quinone sensing and response repressor (QsrR) is a novel kind of thiol-stress-sensing regulator YodB family transcriptional regulator found in the notorious pathogen <em>Staphylococcus aureus</em>. [2] It specifically binds to a palindromic DNA sequence, blocking RNA polymerase and thus repressing transcription. [2] However, with the presence of quinone, it reshapes to bind with quinone molecules and leaves the target DNA, which allows the transcription process to start. [2] Since laccase is used at first to degrade PAHs into quinones for the proposed pathway, the quinone-like compounds produced as intermediates can function as signaling molecules that induce the expression of other enzymes responsible for subsequent degradations. This method can save energy for the cell that expresses those PAH-degrading enzymes. The protein sequence of QsrR is readily available at GenBank, with the reference code 4HQE_A for side chain A, and 4HQE_B for side chain B.</p> | <p>Quinone sensing and response repressor (QsrR) is a novel kind of thiol-stress-sensing regulator YodB family transcriptional regulator found in the notorious pathogen <em>Staphylococcus aureus</em>. [2] It specifically binds to a palindromic DNA sequence, blocking RNA polymerase and thus repressing transcription. [2] However, with the presence of quinone, it reshapes to bind with quinone molecules and leaves the target DNA, which allows the transcription process to start. [2] Since laccase is used at first to degrade PAHs into quinones for the proposed pathway, the quinone-like compounds produced as intermediates can function as signaling molecules that induce the expression of other enzymes responsible for subsequent degradations. This method can save energy for the cell that expresses those PAH-degrading enzymes. The protein sequence of QsrR is readily available at GenBank, with the reference code 4HQE_A for side chain A, and 4HQE_B for side chain B.</p> | ||
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[2] JI, Q., ZHANG, L., JONES, M.B., SUN, F., DENG, X., LIANG, H., CHO, H., BRUGAROLAS, P., GAO, Y.N., PETERSON, S.N., LAN, L., BAE, T. and HE, C., 2013. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proceedings of the National Academy of Sciences, 110(13), pp. 5010-5015.</p> | [2] JI, Q., ZHANG, L., JONES, M.B., SUN, F., DENG, X., LIANG, H., CHO, H., BRUGAROLAS, P., GAO, Y.N., PETERSON, S.N., LAN, L., BAE, T. and HE, C., 2013. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proceedings of the National Academy of Sciences, 110(13), pp. 5010-5015.</p> | ||
<p> </p> | <p> </p> | ||
- | < | + | <h3>T7-RBS-Laccase (Device <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092005">BBa_K1092005</a>)</h3> |
<p>The laccase (cotA) found in <em>Bacillus</em> sp. HR03 is a bacterial laccase that have the potential ability to degrade a variety of aromatic compounds. [3] Previous study showed that it can be successfully expressed in <em>Escherichia coli</em> strain BL-21(DE3), and was correctly folded. [4] Moreover, the complete gene sequence of this laccase was readily recorded in GenBank, with the reference code of FJ663050.1. It is thus used as one of the two major enzymes for PAH degradation.</p> | <p>The laccase (cotA) found in <em>Bacillus</em> sp. HR03 is a bacterial laccase that have the potential ability to degrade a variety of aromatic compounds. [3] Previous study showed that it can be successfully expressed in <em>Escherichia coli</em> strain BL-21(DE3), and was correctly folded. [4] Moreover, the complete gene sequence of this laccase was readily recorded in GenBank, with the reference code of FJ663050.1. It is thus used as one of the two major enzymes for PAH degradation.</p> | ||
<p>Properties of the laccase:<br /> | <p>Properties of the laccase:<br /> | ||
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<p></p> | <p></p> | ||
<p></p> | <p></p> | ||
- | < | + | <h3>FL3-Lgt-FL3 (Protein Domain <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092103">BBa_K1092103</a>)</h3> |
<p>Transmembrane protein Lgt with two flexible linkers FL3 | <p>Transmembrane protein Lgt with two flexible linkers FL3 | ||
This is a biobrick we adapted from SJTU 2012 team, BBa_K771102. | This is a biobrick we adapted from SJTU 2012 team, BBa_K771102. |
Revision as of 15:46, 27 October 2013