Team:Hong Kong CUHK/results

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<h1>Characterization of Laccase</h1>
<h1>Characterization of Laccase</h1>
<h3>1. Biobrick DNA length verification</h3>
<h3>1. Biobrick DNA length verification</h3>
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<p align="center"><img src="https://static.igem.org/mediawiki/parts/7/70/La.jpg" height="400"></p>
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<p align="center"><img src="https://static.igem.org/mediawiki/parts/f/fc/La_2.jpg" height="400"></p>
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<p2><p align="center">Figure 1. Restriction digestion plasmid gel picture</p></p2>
<h3>2. Laccase activity test by automatic kinetic spectrophotometric assay <br>
<h3>2. Laccase activity test by automatic kinetic spectrophotometric assay <br>
using o-phenylenediamine</h3>
using o-phenylenediamine</h3>
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<p>After 10 to 12 hours of incubation at 37 °C, laccase showed maximum activity when 0.3 mM IPTG was used for introducing protein expression (Figure 2).</p>
<p>After 10 to 12 hours of incubation at 37 °C, laccase showed maximum activity when 0.3 mM IPTG was used for introducing protein expression (Figure 2).</p>
<p align="center"><a href="http://parts.igem.org/File:La1.jpg"><img alt="La1.jpg" src="https://static.igem.org/mediawiki/2013/a/a5/800px-La1.jpg" width="600" ></a></p>
<p align="center"><a href="http://parts.igem.org/File:La1.jpg"><img alt="La1.jpg" src="https://static.igem.org/mediawiki/2013/a/a5/800px-La1.jpg" width="600" ></a></p>
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<p align="center">Figure 2. Laccase Activity Monitoring</p>
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<p align="center">Figure 2. Laccase activity monitoring</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<h3>3. Laccase function verification</h3>
<h3>3. Laccase function verification</h3>
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<h3>1. Biobrick DNA length verification</h3>
<h3>1. Biobrick DNA length verification</h3>
<p align="center"><img src="https://static.igem.org/mediawiki/parts/archive/8/8d/20130928021743%21Di.jpg" height="400"></p>
<p align="center"><img src="https://static.igem.org/mediawiki/parts/archive/8/8d/20130928021743%21Di.jpg" height="400"></p>
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<p align="center">Figure 4. Restriction Digestion Gel Photo</p>
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<p align="center">Figure 4. Restriction digestion plasmid gel picture</p>
<h3>2. Catechol 1,2-dioxygenase function verification by time series degradation assay</h3>
<h3>2. Catechol 1,2-dioxygenase function verification by time series degradation assay</h3>
<p>Catechol 1,2-dioxygenase is resposible for phenol degredation (Naiem <em>et al</em>., 2011). Before it is integrated into our PAHs degrdation system, hydroquinone, also known as benzene-1,4-diol or quinol, which is an aromatic organic compound belonging to the phenol family, was used as a substrate to test Catechol 1,2-dioxygenase expression and activity.</p>
<p>Catechol 1,2-dioxygenase is resposible for phenol degredation (Naiem <em>et al</em>., 2011). Before it is integrated into our PAHs degrdation system, hydroquinone, also known as benzene-1,4-diol or quinol, which is an aromatic organic compound belonging to the phenol family, was used as a substrate to test Catechol 1,2-dioxygenase expression and activity.</p>

Latest revision as of 03:52, 29 October 2013

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