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Team:INSA Toulouse/contenu/lab practice/notebook/calendar/dry lab
From 2013.igem.org
Notebook
Dry Lab Calendar
June 2013
- Week 1 (10-16 June)
During this week litterature searches about biological logic gates and protocols were performed.
We also began to plan the lab work for the following weeks. We started to take an inventory of necessary biobricks already available in plates supplied by the MIT and to mail former iGEM teams to receive the others.
Project complet scheme elaboration with all the needeed biobricks. - Week 2 (17-23 June)
We wondered about the use of another strain DH5-1, with a faster growth and so a reduction of waiting times. Reflexions were also performed about the integration strategy of our system in the bacterial host E. coli.
In parallel, we finalized the final global diagram of our system and intermediate constructions for characterizations (light sensors, carry diffusion (AHL) and general inducer (atC)). These ones have been divided up between team members to start first clonings.
New search and amplification of biobricks with rbs and terminators if possible. - Week 3 (24-30 June)
Design of sequences to synthesize in order to save time : logic gates and our riboswitch regulation system in biobrick format.
A new idea for logic gates design came up : recombinases can turn terminators over , instead of genes themselves. So we had to find adapted terminators in the registry part, that is to say very efficient ones in both ways of transcription.
As far as the integration in concerned, reflexion keeps going. The final host can not have more than two plasmids, with two different markers and origins of replication. AND1/AND2 gates should be on a first plasmid and XOR1/XOR2 on an other to reduce recombination problems. Moreover a part of our system has to be integrated in the host’s genome. We did a research about this topic to understand how integrative vectors work. We asked the UNIPV Pavia Team (2010) about their integrative vector for E. coli. Yet, a pir+ strain is needed to make it works... Search has to be continued...
July 2013
- All month
Modeling
Website / Logo
Ethics
Conferences and meetings : Meeting with Mr Cornet about the integration strategy.
August 2013
- All month
Modeling: End of the Chromobacterium experiences, it is now time to model it!
Modelling of diffusion of AHL into LB solid medium with the results of Chromobacterium experiences.
Website / Logo : Every pages have been created. It is now time to complete them!
Ethics: Researches on ethical problem about synthetic biology and molecular biology in general
Conferences and meetings.
September 2013
- Week 13 (1-8 September)
Modeling: Modelling of diffusion of AHL into LB solid medium with the results of Chromobacterium experiences.
Website / Logo
Ethics
Conferences and meetings - Week 14 (9-15 September)
Modeling: Modelling of diffusion of AHL into LB solid medium with the results of Chromobacterium experiences.
Website / Logo: Wiki ready to be completed
Ethics
Conferences and meetings - Week 15 (16-22 September)
Modeling: Modelling of diffusion of AHL into LB solid medium with the results of Chromobacterium experiences.
Website / Logo
Ethics
Conferences and meetings: presentation of the project and the summer's achievements to the LISBP. - Week 16 (23-29 September)
Modeling: Modelling of diffusion of AHL into LB solid medium with the results of Chromobacterium experiences.
Website / Logo
Ethics
Conferences and meetings
October 2013
- Week 17 (30-6 October)
Modeling: Modelling of diffusion of AHL into LB solid medium with the results of Chromobacterium experiences.
Website : On the wiki everyday, last pages, last modifications!!! End is close…. Ethics
Conferences and meetings - Week 18 (7-13 October)
Modeling
Website / Logo: Wiki ready to be completed
Ethics
Conferences and meetings
