Team:KAIST Korea

From 2013.igem.org

(Difference between revisions)
Line 982: Line 982:
<img src="https://static.igem.org/mediawiki/2012/e/e1/KAIST_lionLion.png" style="float:left;padding:10px 20px 10px 0px;margin:0px 0px 20px 0px;"/>
<img src="https://static.igem.org/mediawiki/2012/e/e1/KAIST_lionLion.png" style="float:left;padding:10px 20px 10px 0px;margin:0px 0px 20px 0px;"/>
</br>
</br>
-
<span id="little"><span id="starter">W</span>hat we see and handle in lab is mostly ‘microscopic’. But there are macroscopic stuffs out there! At night, we can see stars, and among them, we can find Lion’s heart, ‘Regulus’. Although it is not the brightest kind, Regulus still makes it easy to notice the Lion in the sky. </span></br></br>
+
<span id="little"><span id="starter">T</span>he widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
-
<span id="little2">Like Regulus, we want our module to be helpful for others to find their own Lion in this universe of synthetic biology. Our module may not be magnificent as that of other teams but we don’t actually want to excel others. We rather want to facilitate the advance of synthetic biology. Like the heart which beats in autonomous manner, our module will run by itself. Regarding all these aspects, we call our project <span style="color:#ff0000;"><b>‘Reguli’</b></span>.</br></br></span>
+
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
 +
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
 +
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
 +
<span style="color:#ff0000;"><b>‘Reguli’</b></span>.</br></br></span>
<div clear='both'></div>
<div clear='both'></div>

Revision as of 12:31, 16 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:KAIST Korea/Project Background - 2013.igem.org

 

Team:KAIST Korea/Project Background

From 2012.igem.org

KAIST Korea 2013 iGEM

Project : Overview

Overview



The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation. iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes. Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control. As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated. ‘Reguli’.



Kaist Footer

Retrieved from "https://2012.igem.org/Team:KAIST_Korea/Project_Background"

Retrieved from "http://2013.igem.org/Team:KAIST_Korea"